Deep-Sea Risk Assessment and Species Sensitivity to WAF, CEWAF and Dispersant

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers 2 (ADDOMEx-2)

Passive Gulf of Mexico Loop Current Observations from High Frequency Radar Across the Yucatan Strait

Description: This project will procure, install, and operate high frequency radar systems to measure surface currents at two locations in the Yucatan Channel region of the Gulf of Mexico: Isla Contoy and Puerto Morelos. Data from the systems will provide new, real-time data for model assimilation and validation to better understand the evolution of the LCS.

Gulf Consortium for Offshore Risk Reduction Engaging Stakeholders (GulfCORES)

Microbial EPS production in response to oil spills; Prediction of marine aggregate’s exposure to chemically enhanced (COREXIT) water accommodated fraction of oil using Fourier Transform Infrared (FTIR) spectroscopy

Dataset for: The impact of dissolved inorganic nitrogen and phosphorous on responses of microbial plankton to the Texas City “Y” oil spill in Galveston Bay, Texas

The carbon budget for microbial EPS production in response to oil and Corexit for COAST, a mesocosm of coastal water with COASTal microbial concentrate

Coastal water with coastal microbial concentrate (COAST): Microbial cell and micro-aggregate counts with parallel beta-galactosidase activity measurements

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Chaetoceros and natural bacterial inoculum

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Thalassiosira pseudonana culture and natural bacterial inoculum

Test of Coastal water with coastal microbial concentrate (TeCOAST): Production of TEP as a function of oil

Coastal water with coastal microbial concentrate (COAST): Production of TEP and CSP as a function of oil

GOM open ocean (GOMOO): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil

GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil

Impact of Corexit on aggregation of young Chaetoceros: Rolling table experiment to test the aggregation of diatoms, TEP (transparent exopolymers), POC (particulate organic carbon) as a function of oil and oil+Corexit

Impact of Corexit on aggregation of old Chaetoceros: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC(particulate organic carbon) as a function of oil and oil+Corexit

Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over time

Impact of Corexit on aggregation of Skeletonema grethae or natural particles in oil contaminated natural seawater, low oil concetrations: Rolling table experiment to test the aggregation of diatoms, TEP (transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over time

Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h

EPS mechanisms which aid in aggregation: A test of light to induce bacterial EPS aggregation, particle size of the EPS solution; protein and carbohydrate composition of aggregates and colloidal filtrate

Photographs of marine phytoplankton exposed to crude oil and dispersants in mesocosm tanks after 72h

Oil Composition for the TeCOAST mesocosm study, Test of COASTal water with coastal microbial concentrate

Oil Composition and nutrients for the COAST mesocosm study, COASTal water with added coastal microbial concentrate

Response of oil-degrading microbes to dispersants and how dispersants affect the ternary system (oil-dispersant-EPS): from TeCOAST, Test of Coastal water with coastal microbial concentrate, DOM FTICR MS data

Response of oil-degrading microbes to dispersants in a ternary system (oil-dispersant-EPS): TeCOAST, a mesocosm Test of COASTal water with microbial concentrate, Solid-state 13C NMR data for EPS

Response of oil-degrading microbes in a ternary system (oil-dispersant-EPS): COAST, coastal water mesocosm with microbial concentrate, solid-state 13C NMR data

p450 gene abundance measurements in the COAST mesocosm study

Three dimensional micrographs of microbe-oil microaggregates from the TeCOAST and COAST mesocosms

Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters

Response of oil-degrading microbes in the ternary (oil-dispersant-EPS) system: TeCOAST, Test of Coastal water with coastal microbial concentrate, bottom particle dichloromethane extract FTICR MS data

Response of oil-degrading microbes in the ternary (oil-dispersant-EPS) system: COAST, mesocosm experiment with Coastal water with coastal microbial concentrate, bottom particle dichloromethane extract FTICR MS data

The microbial EPS production and composition in response to oil and Corexit for COAST, a mesocosm of COASTal water with COASTal microbial concentrate

The effect of Oil and Corexit on Marine Phytoplankton Exopolymeric Substances Release

Impact of Oil Spill on Marine Microgel Aggregation Process

GC MS data for bottom particle dichloromethane extracts from the COAST, a COASTAL water mesocosm experiment amended with microbial concentrate

Long Term Marine Oil Snow Experiment (LTMOSE): TEP as a function of time

The effects of sunlight on the composition of exopolymeric substances affecting the subsequent aggregation process during oil spill and Corexit exposure

Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Mixture (t = 4 days)

Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours)

Component-specific Investigation of Oil Distribution in Seawater - Partitioning of oil in the absence of biota : Control tank data after 1 hour

Extracellular Enzyme Activity Profile in Chemically Enhanced Water Accommodated Fraction of Surrogate Oil from BP Oil Spill

Oil Composition and nutrients for the GOMOO mesocosm using Gulf Of Mexico Open Ocean water

Oil Composition and nutrients for the GOMCOAST mesocosm using Gulf Of Mexico COASTal water

Chemical Composition of WAF made through a novel passive dosing method tested during the GOMCOAST mesocosm using Gulf Of Mexico COASTal water

Photographs of aggregates in the mesocosm COASTal water amended with COASTal microbial concentrate

Percent relative abundance of the eukaryotic microbial community in the GOMCOAST (Gulf of Mexico, Coastal Water) mesocosm from 8 micron polycarbonate filters

Production of extracellular polymeric substances (EPS) and hydrocarbon degradation of bacteria isolated from the northern Gulf of Mexico surface water

Light induced aggregation of protein-containing compounds

Particle size of light induced aggregates using flow cytometry

GOM open ocean (GOMOO): Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit

GOM coastal waters (GOMCOAST): Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit

Surface tension of exopolymeric substances in seawater in the presence or absence of oil and/or Corexit

Intercalibration of the Estimated Oil Equivalence (EOE) of WAFs and CEWAFs using 3 different spectrofluorometers

Bulk chemistry of aggregates and EPS for roller table experiments for diatom aggregation in the presence or absence of oil

Molecular level characterization of oil and aggregate oxidation products: Mesocosm LTMOSE, a Long Term Marine Oil Snow Experiment, Fourier transform ion cyclotron mass spectrometry (FTICR-MS) data

Molecular level characterization of oil and aggregate oxidation products: Mesocosm LTMOSE, a Long Term Marine Oil Snow Experiment, Nuclear magnetic resonance (NMR) data

Oil concentration, oil composition, biomarkers, and nutrients for the mesocosm LTMOSE, a Long Term Marine Oil Snow Experiment

Marine gels, extracellular polymeric substances (EPS) and transparent exopolymeric particles (TEP) in natural seawater and seawater contaminated with a water accommodated fraction of Macondo oil surrogate

Petrocarbon incorporation into marine snow in three mesocosm experiments and the exopolymeric substances (EPS) composition

Microbial community dynamics in the Flower Garden Banks National Marine Sanctuary (FGBNMS) after the summer 2016 mass invertebrate mortality event, 2016-07-31 to 2016-08-01

Microbial community response in non-axenic cultures of Thalassiosira pseudonana to crude oil with and without the chrysolaminarin synthesis inhibitor dichlorobenzonitrile

Biological and physiological responses during 14 day incubations of 15 phytoplankton species in culture exposed to oil and combinations of oil and dispersant

Microbial species growth and exopolymeric substance production microchip screening test

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using the diatom Odontella

EPS mechanisms which aid in aggregation: A test of light to induce bacterial EPS aggregation, turbidity, aggregate weight, FTIR, DOC and DN, and UV-Vis

EPS mechanisms which aid in aggregation: Light-induced aggregation of bacterial exopolymeric substances using DLS

Impact of Oil and Corexit on Marine Dissolved Organic Matter (DOM) Aggregation and Microgel Formation Through Dynamic Laser Scattering (DLS)

Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit in Coastal Water Mesocosms

Scanning Electron Microscopy (SEM) observation of morphologies of sediments from a mesocosm experiment studying formation of marine aggregates with oil and dispersant

Fluorescence Enhancement Measurement of hydrophobic components and polysaccharides in sediment exposed to oil and dispersant

False-colored photographs of micro-scale microbial aggregates collected from four different mesocosm treatments after 48 hours

Raw and processed negative electrospray ionization coupled to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) data for mesocosm experiment samples collected from bulk DOM tank, control tank marine snow, and WAF tank marine snow.

Photographs of microbial aggregates with and without oil or Corexit collected from four different mesocosm treatments

Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016

Eukaryotic community response to oil and Corexit in a coastal water mesocosm, August 2015

Eukaryotic community response to oil and Corexit in a mesocosm, October 2015

Estimated Oil Equivalence (EOE) of Water Accommodated Fraction (WAF) made through three different procedures

Chemical composition of Water Accommodated Fraction (WAF) made through three different procedures

Toxicity of 1-methylnaphthalene to Americamysis bahia for use in the target lipid model

DETOX 1: Concentration of 1-methyl naphthalene in dosing chambers of deep sea organisms

Toxicity of 1-methylnaphthalene to five deep-sea micronekton species

Partitioning coefficient of 1-methyl naphthalene and phenanthrene at various temperature and salinity

Phenanthrene Toxicity to Americamysis bahia and 5 deep-sea crustaceans

Chemical composition of WAF made with traditional CROSERF method and two passive dosing methods and comparison with predicted concentrations from PETROTOX model

MC252 Oil Toxicity to Americamysis bahia, Lysmata boggessi, and two Deep-Sea Crustaceans

Toxicity of chemically enhanced water accommodated fraction (CEWAF) of MC252 crude oil and Corexit 9500 to 4 deep-sea crustaceans

Acute toxicity of chemically enhanced water accommodated fraction (CEWAF) to deep-sea crustaceans and peppermint shrimp (Lysmata wurdemanni)

Adhesive force measurements between extracellular polymeric substances (EPS)

Hydrophobic droplets trigger secretion of protein-rich microbial extracellular polymeric substances from phytoplankton

Exopolymeric substances (EPS) mechanisms in aggregation & dispersion of oil: Fragmentation of Skeletonema grethae-775 snow – Aggregate characteristics

Exopolymeric substances (EPS) mechanisms in aggregation & dispersion of oil: Fragmentation of Skeletonema grethae-775 snow – Disaggregation dynamics

Analytical measurements: Estimated Oil Equivalents (EOE) for MICROX, a mesocosm study to quantify microbial oxidation and degradation of oil

Analytical measurements: Time series of nutrients in MICROX, a mesocosm to quantify microbial oxidation and degradation of oil

Microbial Community Composition and Structure, in MICROX, a mesocosm for studying the microbial oxidation and degradation of oil, June 2018, 16S

Microbial Community Gene Expression, Mixed Isolate Bottle Experiment, LTOPOS, a Long Term Oil and Particle Oxidation mesocosm Study, qPCR measurements

Mesocosom: Molecular level characterization of oil and aggregate oxidation products: MICROX, microbial oxidation and degradation of oil study (June 2018), FTICR-MS data

Molecular level characterization of oil and aggregate oxidation products: MICROX, a mesocosm for studying microbial oxidation and degradation of oil, two-dimensional gas chromatography-mass spectrometry (GCxGC-MS) data

Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments

Change in molecular weight of aggregates in artificial seawater formed during irradiation and during irradiation with reactive oxygen species (ROS), size fractions of organic carbon

Mesocosm: Rates of 14C hydrocarbon oxidation by microbial communities, MICROX, microbial oxidation and degradation of oil and oxidation rate measurements

Mesocosm: Characterization of cell concentrations and microaggregate abundance, MICROX, to study the microbial oxidation and degradation of oil

Mesocosm: Fluorescent in situ hybridization of microaggregates, LTMOSE, a long-term marine oil snow experiment, abundances of Alcanivorax and Alteromonas

Mesocosm: microbial community structure, MICROX, microbial oxidation and degradation of oil, SSU rRNA sequences

Microaggregate microbial community metagenome, LTMOSE, a Long Term Marine Oil Snow Mesocosm Experiment, sequence data

Microcosm: Generation of EPS by phytoplankton and bacteria with and without polysaccharide inhibitor at various concentrations of WAF

Microcosm: Flask experiment of phytoplankton-bacteria interaction in a natural community using control and oil treatments; non-radioactive experiment; extracellular polymeric substances (EPS) assays for protein, neutral sugar, uronic acid, organic carbon, and total nitrogen

Microcosm: Flask experiment of phytoplankton-bacteria interaction in a natural community using control and WAF treatments; radiolabeling experiment; activities of bacteria, phytoplankton, oil

Microcosm: SEM micrographs of generation of exopolymeric substances (EPS) by phytoplankton and bacteria with and without polysaccharide inhibitor at various concentrations of WAF

Microcosm: CLSM micrographs of generation of exopolymeric substances (EPS) by phytoplankton and bacteria with and without polysaccharide inhibitor at various concentrations of WAF

Microcosm: Flask experiment of phytoplankton-bacteria interaction in a natural community using control and water-accommodated fraction (WAF) treatments; non-radioactive experiment; Imaging FlowCytobot

Microcosm: Flask experiment of phytoplankton-bacteria interaction in a natural community using control and water accommodated fraction (WAF) treatments; non-radioactive experiment, 16s sequencing

Microaggregate Microbial Community Structure, Mesocosm Experiment, LTMOSE, a Long Term Marine Oil Snow Mesocosm Experiment, SSU rRNA sequences

Proteolysis rates and carbonyl contents of model proteins (fetuin, cytochrome c, bovine serum albumin, and lectin) during sunlight irradiation

Hydroxyl radical formation rates during irradiation of hydrogen peroxide in artificial seawater

Response of toxigenic Pseudo-nitzschia sp. to oil and dispersant exposure over 6 days

Dataset for: Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum

Phyto-PAM-Fluorescence data: Effect of water-accommodated fraction (WAF) and water-soluble fraction (WSF) on phytoplankton’s photosynthetic performance and their relative abundance

Molecular level characterization of oil and aggregate oxidation products: LTOPOS, a Long Term Oil and Particle Oxidation Study, two-dimensional gas chromatography-mass spectrometry (GCxGC-MS) data

Molecular level characterization of oil and aggregate oxidation products: LTOPOS, a Long Term Oil and Particle Oxidation Study, Fourier transform ion cyclotron mass spectrometry (FTICR-MS) data

Eukaryotic phytoplankton community composition in the COAST (Gulf of Mexico, COASTal water mesocosms amended with coastal microbial concentrate) mesocosm as identified by 18S sequence from RNA-seq whole transcriptome sequencing for four treatments (control, WAF, CEWAF, dilute CEWAF)

Analytical measurements of nutrients in a mesocosm, a Long Term Oil and Particle Oxidation Study (LTOPOS)

Estimated Oil Equivalents (EOE) for LTOPOS, a mesocosm to quantify microbial oxidation and degradation of oil

Rates of 14C hydrocarbon oxidation by microbial communities, LTMOSE, a Long Term Marine Oil Snow Experiment, oxidation rate measurements, a mesocosm experiment

Characterization of cell concentrations and microaggregate abundance and size, LTMOSE, a Long Term Marine Oil Snow Mesocosm Experiment, abundance counts

Metatranscriptomes: Gulf of Mexico Open Ocean (GOMOO) and Gulf of Mexico Coastal Waters (GOMCOAST)

Concentrations of polycyclic aromatic hydrocarbons (PAHs) in surface water of Galveston Bay collected aboard R/V Trident cruises following Hurricane Harvey from 2017-09-04 to 2017-09-28

ADDOMEx Tier 3 Experiments: Mesocosm #3 Gulf of Mexico open ocean waters (GOMOO) and Mesocosm #4 Gulf of Mexico coastal waters (GOMCOAST) 10 um filter size

Mesocosm experiment: Exoenzyme activity, organic carbon and nitrogen, exopolymeric substances, dissolved inorganic nitrogen and phosphate, and estimated oil equivalent data measured using control and WAF treatments

ADDOMEx Tier 3 Experiments: Mesocosm #3 Gulf of Mexico open ocean waters (GOMOO) and Mesocosm #4 Gulf of Mexico coastal waters (GOMCOAST) 0.2 um filter size

Concentrations of polycyclic aromatic hydrocarbons (PAHs) in marine snow aggregates

How does oil exposure affect centric diatom Thalassiosira pseudonana?

Identification of biochemical pathways contributing to oil tolerant ability of Dunaliella tertiolecta

Response of Karenia brevis growth and brevetoxin production to oil and dispersant exposure

Impacts of photo-oxidized dispersed oil on Phaeodactylum tricornutum

The effect of oil and weathered oil along with nitrogen and carbon supplementation on natural phytoplankton community composition, growth and physiology

Potential impacts of ocean acidification on diatom aggregation when exposed to crude oil and chemical dispersants

Effects of nutrient availability on the diatom Phaeodactylum tricornutum in the presence of oil

Molecular level characterization of oil and aggregate oxidation products: LTOPOS, a Long Term Oil and Particle Oxidation Study, Nuclear magnetic resonance (NMR) data

Alkanes Data in Seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (WAF) of Oil for LTOPOS, a Long-Term Oil and Particle Oxidation Study (Mesocosom 7)

Targeted metabolite data in seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (DCEWAF) of Oil for LTOPOS a Long Term Oil and Particle Oxidation Study (Mesocosom 7)

Mesocosm 7: Polycyclic Aromatic Hydrocarbon (PAH) data in seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (DCEWAF) of oil for LTOPOS, a Long Term Oil and Particle Oxidation Study

Exopolymeric substances (EPS) composition in colloidal fraction, suspended particulate matter and sinking marine snow or marine oil snow during a long term coastal phytoplankton seeded mesocom experiment

Colored dissolved organic matter (CDOM) and chlorophyll a and b data in seawater, water-accommodated fraction (WAF) and diluted chemically enhanced WAF (DCEWAF) of oil for the mesocosm LTMOSE, a Long Term Marine Oil Snow mesocosm Experiment

Dataset for: Microbial, Physical, and Chemical Changes in Galveston Bay Following an Extreme Flooding Event, Hurricane Harvey

Dataset for: A ribosomal sequence-based oil sensitivity index for phytoplankton groups

Effect of oil exposure on reactive oxygen species (ROS) levels in diatom Thalassiosira pseudonana and a comparison of exposure of oil to hydrogen peroxide on the photosynthetic physiology within 24 hours

Reactive oxygen diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana in response to oil exposure

Polycyclic Aromatic Hydrocarbons (PAHs) Cycling and Fates in Galveston Bay, Texas, USA

Mesocosm: Eukaryotic community response to oil and oil-dispersant mixtures in the COAST (Gulf of Mexico, COASTal water mesocosms amended with coastal microbial concentrate)