Abstract:

Mesocosm experiments were performed with seawater collected from Galveston Bay, Texas. Polycyclic Aromatic Hydrocarbon (PAH) were measured in filtered samples from two treatments: 1) control (seawater) and 2) Diluted Chemically-Enhanced Water-Accommodated Fraction (DCEWAF) of crude oil. PAHs from two fractions were collected: particle associated and dissolved. The extracted samples were analyzed on gas chromatography-mass spectrometry (GC-MS).

Suggested Citation:

Liu, Yina and Terry Wade. 2020. Mesocosm 7: Polycyclic Aromatic Hydrocarbon (PAH) data in seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (DCEWAF) of oil for LTOPOS, a Long Term Oil and Particle Oxidation Study. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/ER1EGZ8E

Purpose:

To determine the concentration of Polycyclic Aromatic Hydrocarbon (PAH) during microbial oxidation of crude oil components.

Data Parameters and Units:

PAH (Polycyclic Aromatic Hydrocarbon); DCEWAF (Diluted Chemically Enhanced Water Accommodated Fraction); Naphthalene, 2-Methylnaphthalene, 1-Methylnaphthalene, C2-Naphthalenes, C3-Naphthalenes, C4-Naphthalenes, Biphenyl, 2,6-Dimethylnaphthalene, Acenaphthylene, Acenaphthene, 1,6,7-Trimethylnaphthalene, Fluorene, C1-Fluorenes, C2-Fluorenes, C3-Fluorenes, Dibenzothiophene, C1-Dibenzothiophenes, C2-Dibenzothiophenes, C3-Dibenzothiophenes, Phenanthrene, Anthracene, C1-Phenanthrenes/Anthracenes, C2-Phenanthrenes/Anthracenes, C3-Phenanthrenes/Anthracenes, C4-Phenanthrenes/Anthracenes, 1-Methylphenanthrene, Fluoranthene, Pyrene, C1-Fluoranthenes/Pyrenes, C2-Fluoranthenes/Pyrenes, C3-Fluoranthenes/Pyrenes, Benzo(a)anthracene, Chrysene, C1-Chrysenes, C2-Chrysenes, C3-Chrysenes, C4-Chrysenes, Benzo(b)fluoranthene, Benzo(k)fluoranthene, Benzo(e)pyrene, Benzo(a)pyrene, Indeno(1,2,3-c,d)pyrene, Dibenzo(a,h)anthracene, Benzo(g,h,i)perylene, Perylene. Units: ng/L

Methods:

Polycyclic Aromatic Hydrocarbon (PAH) samples were collected for both particle associated phase and dissolved phase. Dissolved phase was collected for each sample passing through a filtration series (GF/D and then 0.2µm). The particle associated phase is defined as particles stayed on the GF/D and 0.2 µm filters. The dissolved phase for each sample was preserved in dichloromethane and extracted with liquid-liquid extraction with dichloromethane. The particle associated phase (i.e. filters) samples were stored at -20⁰C before extraction. The filters for each sample (GF/D and 0.2 µm) were combined and extracted with dichloromethane. The extracted samples were analyzed on gas chromatography-mass spectrometry (GS-MS). Particle associated phase concentrations were normalized to the volumes of water passed through the filters.

Instruments:

gas chromatography-mass spectrometry (GS-MS)

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