Abstract:

Diatoms secrete a significant amount of polysaccharides into the environment, which can serve as a critical organic carbon source for bacteria. These polysaccharides (especially chrysolaminarin) are produced in copious amounts in diatoms especially during the stationary phase of their growth. The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that had significantly impacted the phytoplankton community. Here, we investigated the microbial community response in non-axenic cultures of an oil sensitive diatom species, Thalassiosira pseudonana (CCMP 1335), with and without the chrysolaminarin synthesis inhibitor Dichlorobenzonitrile (DCB), and in the presence and absence of oil.

Suggested Citation:

Shawn Doyle, Manoj Kamalanathan, Hernando Bacosa. 2019. Microbial community response in non-axenic cultures of Thalassiosira pseudonana to crude oil with and without the chrysolaminarin synthesis inhibitor dichlorobenzonitrile. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-7vhe-m749

Publications:

Kamalanathan, M., Chiu, M.-H., Bacosa, H., Schwehr, K., Tsai, S.-M., Doyle, S., … Quigg, A. (2019). Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence. Plant Physiology, 180(4), 1898–1911. doi:10.1104/pp.19.00301

Purpose:

To investigate the microbiome response of non-axenic cultures of Thalassiosira pseudonana (CCMP 1335) with and without the chrysolaminarin synthesis inhibitor Dichlorobenzonitrile (DCB), and in the presence and absence of oil.

Data Parameters and Units:

SRA run info table provided from the National Center of Biotechnology Information (NCBI). Experimental information and SRA accession numbers for raw sequence read located at NCBI.

Individual Files: