Abstract:

Photo-oxidation experiments on several model proteins (fetuin, cytochrome c, bovine serum albumin (BSA), and lectin) in artificial seawater were carried out to investigate the mechanisms for the transformation and preservation of proteinaceous compounds. TOC (total organic carbon) of model proteins during sunlight irradiation was examined.

Suggested Citation:

Sun, Luni and Peter Santschi. 2020. Change in molecular weight of aggregates in artificial seawater formed during irradiation and during irradiation with reactive oxygen species (ROS), size fractions of organic carbon. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-bxec-x617

Purpose:

To investigate the mechanisms for the transformation and preservation of proteinaceous compounds in artificial seawater.

Data Parameters and Units:

TOC=total organic carbon, parts per million (ppm); irradiation time: 0 minutes, 15 minutes, 2 hours, 6 hours, 20 hours; No H2O2= with no H2O2 addition; H2O2=with H2O2 addition; BSA= bovine serum albumin

Methods:

Model proteins (30 mg/L) were dissolved in artificial seawater and irradiated in quartz vials (125 mL) under a 400W xenon lamp (SUNTEST system in a circulating water bath). H2O2 (in the mass ratio of 1:1 to protein) was added into half of the samples. Subsamples were taken at 0 minutes, 15 minutes, 2 hours, 6 hours, and 20 hours intervals. After 0.4 µm filtration, the subsamples were concentrated by ultrafiltration to obtain the colloidal fraction using an Amicon ultra-4 centrifugal filter unit with a 3kDa cut-off membrane. The concentrations of organic carbon were measured on a Shimadzu Total Organic Carbon-L analyzer.

Instruments:

Shimadzu TOC-L analyzer (Total Organic Carbon); SUNTEST system

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