Abstract:

Species-level variability has made it challenging to establish the relative sensitivity of phytoplankton to oil and mixtures of oil and dispersant. In this research, a phytoplankton group sensitivity index using ribosome sequence data was developed and was applied to a mesocosm experiment in which a natural microbial community was exposed to oil and two oil-dispersant mixtures. Using the log of the ratio of the number of species that increase to the number that decrease in relative abundance in the treatment relative to the control, the relative sensitivity of four phytoplankton taxonomic groups, diatoms, dinoflagellates, green algae, and Chrysophytes, was computed. This dataset supports the publication: Finkel, Z. V., Liang, Y., Nanjappa, D., Bretherton, L., Brown, C. M., Quigg, A., & Irwin, A. J. (2020). A ribosomal sequence-based oil sensitivity index for phytoplankton groups. Marine Pollution Bulletin, 151, 110798. doi:10.1016/j.marpolbul.2019.110798

Suggested Citation:

Andrew Irwin. 2020. Dataset for: A ribosomal sequence-based oil sensitivity index for phytoplankton groups. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/DDESWY3N

Publications:

Finkel, Z. V., Liang, Y., Nanjappa, D., Bretherton, L., Brown, C. M., Quigg, A., & Irwin, A. J. (2020). A ribosomal sequence-based oil sensitivity index for phytoplankton groups. Marine Pollution Bulletin, 151, 110798. doi:10.1016/j.marpolbul.2019.110798

Purpose:

To characterize the microbial community composition and structure in the mesocosm.

Data Parameters and Units:

The worksheet "Table 1" contains the following: Phytoplankton group: Species are assigned to one of four groups Treatment: One of three treatments (WAF, DCEWAF, CEWAF) analyzed relative to the control Total species: The number of species in each combination of group and treatment (with comparison possible to control) Increase: the number of species increasing in relative abundance (95% confidence interval in brackets) Decrease: the number of species decreasing in relative abundance (95% confidence interval in brackets) Significant Increase: the number of species increasing in relative abundance, as selected by a t-test with false discovery rate correction (95% confidence interval in brackets) Significant decrease: the number of species decreasing in relative abundance, as selected by a t-test with false discovery rate correction (95% confidence interval in brackets) Sensitivity index The worksheet "Accession codes" contains the following: Treatment (WAF, DCEWAF, CEWAF, Control) BioProject = PRJNA489497 Replicate = 1-3 for different treatment BioSample-accession number Run-accession number Please note: WAF = water accommodated fraction of oil; CEWAF = chemically enhanced water accommodated fraction of dispersant and oil; DCEWAF = diluted CEWAF.

Methods:

For detail methods, please refer to the associated publication Finkel et al., 2020.

Individual Files: