Abstract:

The dataset includes vial tests to investigate how phytoplankton extracellular polymeric substance (EPS) influences the growth and production of a marine phytoplankton species. Commercial kit assays based on a small-volume 96-well ELISA platform (Nunc MaxiSorp, VWR, CA, USA) were applied for cellular DNA, protein and ConA-specific carbohydrate determination, to provide an assessment of remaining viable cells and secreted EPS of tested microbial species in response to nano- and microplastics.

Suggested Citation:

Ruei-Feng Shiu, Wei-Chun Chin. 2020. Hydrophobic droplets trigger secretion of protein-rich microbial extracellular polymeric substances from phytoplankton. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-3axn-rr89

Publications:

Shiu, R.-F., Vazquez, C. I., Chiang, C.-Y., Chiu, M.-H., Chen, C.-S., Ni, C.-W., … Chin, W.-C. (2020). Nano- and microplastics trigger secretion of protein-rich extracellular polymeric substances from phytoplankton. Science of The Total Environment, 748, 141469. doi:10.1016/j.scitotenv.2020.141469

Purpose:

Testing microbial EPS production under various concentrations of hydrophobic droplets.

Data Parameters and Units:

Extracellular polymeric substance (EPS) 6 μm/1 μm/ 55 nm are microbead sizes (using hydrophobic microbeads as surrogate of hydrophobic droplets) DNA-Mean and DNA_SD represents the mean and standard deviation of phytoplankton relative DNA amount when exposed to different sized microbeads, respectively. P/C-Mean and P/C_SD represents the mean and standard deviation of phytoplankton relative protein/carbohydrate ratio amount when exposed to different sized microbeads, respectively.

Methods:

Commercial kit assays based on a small-volume 96-well ELISA platform (Nunc MaxiSorp, VWR, CA, USA) were applied for cellular DNA, protein and ConA-specific carbohydrate determination, to provide an assessment of remaining viable cells and secreted EPS of tested microbial species in response to nano- and microplastics. Briefly, an aliquot of phytoplankton suspension (150 μL) was pipetted into the 96-well ELISA plates (Nunc MaxiSorp, VWR, CA, USA) and pre-incubated for 24 h, to enhance the microbe stability in microplates. After that, a serial concentration gradient of the three sized spheres were added into a 96-well plate. Phytoplankton cells were treated with a serial concentration gradient of the three sized PS spheres: Control (without spheres), 10-4, 10-3, 10-2, 10-1, 1, 10, 50, 250 mg L-1 respectively. After the exposure for 48 h, we used plate centrifuge (Megafuge 1.0R; Hereaus, Osterode, Germany) to separate pellet and supernatant phases (405 rad/sec, 5 mins). The living and dead cells usually are concentrated within the pellet and the supernatant phase contain EPS after centrifugation. The pellet was then processed for remaining viable cells (cellular DNA) and the supernatant (EPS) was collected for the secretion composition of the isolates (proteins and carbohydrates).

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