Abstract:

The dataset includes microfluidics, adhesive force measurements and magnetic field-particle experiments to investigate the mechanism of extracellular polymeric substances (EPS) particle aggregation.

Suggested Citation:

Ruei-Feng Shiu, Wei-Chun Chin. 2020. Adhesive force measurements between extracellular polymeric substances (EPS). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-bwdt-hs69

Publications:

Chen, C.-S., Shiu, R.-F., Hsieh, Y.-Y., Xu, C., Vazquez, C. I., Cui, Y., … Chin, W.-C. (2021). Stickiness of extracellular polymeric substances on different surfaces via magnetic tweezers. Science of The Total Environment, 757, 143766. doi:10.1016/j.scitotenv.2020.143766

Purpose:

Measuring adhesive forces (stickiness) of microbial EPS under various environmental conditions.

Data Parameters and Units:

atomic force, N/m Extracellular polymeric substance (EPS) Artificial seawater (ASW) Ethylenediaminetetraacetate (EDTA) Coccolithophore (Cocco) Amphora sp. (Amphora) Sagittula stellata (Sagittula) Standard deviation (SD) Stickiness unit is pico-Newton (pN)

Methods:

Using a magnetic tweezers system, we developed an in vitro method to evaluate the stickiness (adhesive force) of EPS with quantitative force and desired environmental parameters, to understand the early stages of biofilm and marine snow formation. Two parameters, time-lapse and designed force, were applied to measure the stickiness of EPS extracted from Coccolithophore, Amphora sp., and Sagittula stellata.

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