Abstract:

Acute toxicity of Macondo oil (MC252) was tested previously on deep-sea crustaceans. In this dataset, acute toxicity of chemically enhanced water accommodated fraction (CEWAF) was tested against deep-sea crustaceans and also on peppermint shrimp (Lysmata wurdemanni).

Suggested Citation:

Bera, Gopal; Turner, Nick. 2020. Acute toxicity of chemically enhanced water accommodated fraction (CEWAF) to deep-sea crustaceans and peppermint shrimp (Lysmata wurdemanni). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/3V320GBD

Purpose:

Evaluate the toxicity of oil and dispersant on deep-sea crustaceans and proxy crustaceans.

Data Parameters and Units:

Each organism contains the same data parameters/units. Oil Loading (mg/L), Dispersant loading (µg/L), Estimated Oil Equivalents (EOE; µg/L), Total polyaromatic hydrocarbon (TPAH) concentration (µg/L), Temperature (°C), pH, Dissolved Oxygen (mg/L), Number of organisms per chamber (N), Nutrients (mg/L), number dead at each time (h). Chamber = the chamber number, Oil Loading= MC252 oil loaded into each chamber (mg/L), Dispersant= Corexit 9500 loading following oil addition (µg/L), N= number of crustaceans in the chamber. EOE concentrations: T0h, T24h, and T48h= EOE concentrations measured at each time (µg/L). EOE concentrations were measured using an Aqualog Horiba Fluorometer (Model: Aqualog-UV-800). TPAH concentration: T0h and T48h= TPAH concentrations measured at each time (µg/L). TPAH concentrations were measured using gas chromatography with mass spectrometric detection (Agilent 6890N GC/5975C inert MSD) in the selected ion mode (SIM) using a DB-5 fused silica capillary column. Temp48= measured temperature in each chamber at 48h (°C), pH48= pH in each chamber at 48h, DO48= dissolved oxygen in each chamber at 48h (mg/L), PO4= phosphate concentration in each chamber at 48h (mg/L), NH3= ammonia concentration in each chamber at 48h (mg/L), NO2= nitrate concentration in each chamber at 48h (mg/L), NO3=nitrate concentration in each chamber at 48h (mg/L). Chamber = the chamber number, Oil Loading= MC252 oil loaded into each chamber (mg/L), Time from exposure initiation: Each score listed is the number of dead crustaceans in each chamber.

Methods:

Added: Size and Volume of tanks: 750 mL exposure chamber connected to 5 L dosing reservoir, operating experimental water volume 4750 mL Source of crustaceans: Gulf of Mexico Source of water: laboratory system (artificial seawater)

Instruments:

Aqualog Horiba Fluorometer (Aqualog-UV-800) GCMS (Agilent 6890N GC/5975C inert MSD)

Individual Files: