Abstract:
The Deepwater Horizon oil spill spilled 130 million gallons of oil into the Gulf of Mexico. This was followed by application of 1.4 million gallons of chemical dispersants in an effort to disperse the oil into the water column and promote the biodegradation of oil. This dataset was from two flask experiments (short term) using control and oil treatments in triplicate of diatom culture Thalassiosira pseudonana. The experiments were run for a total duration of 48 hours. The parameters measured in the first experiment include cell counts, reactive oxygen levels (ROS), and in ROS/cells. The parameters measured in the second experiment include cell counts, photosynthetic efficiency (Fv/Fm), electron transport rates between photosystem II and I (rETRmax), and light harvesting ability (α), PS II connectivity (ρ), QA turnover rates (τ), and functional absorption cross sectional area (σ).
Suggested Citation:
Kamalanathan, Manoj and Antonietta Quigg. 2021. Effect of oil exposure on reactive oxygen species (ROS) levels in diatom Thalassiosira pseudonana and a comparison of exposure of oil to hydrogen peroxide on the photosynthetic physiology within 24 hours. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/ECPYPXYZ
Data Parameters and Units:
The headers are Sample; Time; Condition; Acetate 0.5 grams per litre; Alpha; Cell counts; Treatment; p; ETRmax; FvFm; Value; ROS; Azide; Sigma; Tau. ROS: Relative fluorescence; ROS/cell: Relative fluorescence/cell; Alpha: micromol e-.micromol-1 photons; ETRmax: micromol.electrons m2.s-1; Fv/Fm: relative fluorescence; Sigma PSII: Å2 (quanta)-1; PS II connectivity: Relative units; QA turnover rates: µsecs
Methods:
Photo-physiological parameters such as maximum quantum yield (Fv/Fm; relative units), relative electron transport rates (rETRmax; µmol electron m-2 s-1), and light harvesting efficiency (α; µmol electrons/µmol photons) were measured on dark acclimated (15 mins) cultures using a Pulse-Amplitude–Modulated Fluorescence System (Phyto-PAM; Walz), operated by the software Phyto-Win (v2.13; Walz). A Fluorescence Induction and Relaxation Fluorometer System (Satlantic) was used to measure functional absorption cross-section area (σPSII; Å2 quanta–1) and QA turnover rates of plastoquinone (τPQ; µs) on dark acclimated (15 mins) cultures.
ROS levels were quantified using modifications of methods described by Rijstenbil, (17). Briefly, 1 mL of samples were stained with 10 mM of 2',7'-dichlorofluorescien diacetate (DCFH-DA) for 60 mins in complete darkness. The samples were then centrifuged at 10,000 g for 5 mins, following which the supernatant was discarded, and the stained cells were re-suspended with fresh f/2 media. The fluorescence was then measured at 488/520 nm excitation/emission using BioTek CytationTM 5 imaging reader controlled by Gen52.09 software (USA).
Growth was monitored by daily cell counts using a Neubauer hemocytometer.
Error Analysis:
For measuring reactive oxygen species (ROS) sample was taken at time 0,2,4,8,24 and 48 hours.
Provenance and Historical References:
Kamalanathan, Manoj, Meng-Hsuen Chiu, Hernando Bacosa, Kathy Schwehr, Shih-Ming Tsai, Shawn Doyle, Alexandra Yard, Savannah Mapes, Carols Vasequez, Laura Bretherton, Jason B. Sylvan, Peter Santschi, Wei-Chun Chin, and Antonietta Quigg. 2019. Role of polysaccharides in diatom Thalassiosira pseudonana and its associated bacteria in hydrocarbon presence. Plant physiology 180(4):1898-1911. DOI: 10.1104/pp.19.00301
Sun, Luni, Meng-Hsuen Chiu, Chen Xu, Peng Lin, Kathleen A. Schwehr, Hernando Bacosa, Manoj Kamalanathan, Antonietta Quigg, Wei-Chun Chin, and Peter H. Santschi. 2018. The effects of sunlight on the composition of exopolymeric substances and subsequent aggregate formation during oil spills. Marine Chemistry 203(20): 49-54. DOI: 10.1016/j.marchem.2018.04.006
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