Abstract:

Pregnant dams were treated at day E11.5 with either 62.5 ug/mL DOSS in drinking water or 0.5% carboxy methyl cellulose (CMC) in drinking water through weaning of pups. Pups were then given normal drinking water until time of sacrifice. Plasma was collected from sacrifice animals and analyzed for adipokine and cytokine levels including Adiponectin, Leptin, Insulin, IL-6 and TNF-alpha. Treated animals exhibit increased IL-6 and TNF-alpha levels in conjunction with decreased Adiponectin levels consistent with a metabolic disruption phenotype and glucose intolerance which was observed in these animals. No changes were observed in plasma leptin and insulin levels, suggesting glucose intolerance is not due to altered insulin levels.

Suggested Citation:

Spyropoulos, D; Temkin, A; Baatz, J. 2017. DOSS Treated Mouse Plasma Adipokine and Cytokine Levels. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7FT8JF6

Purpose:

To test the hypothesis that DOSS exposure in utero alters the metabolic phenotype of newborn mouse pups.

Data Parameters and Units:

Sample Number, Treatment (control- received drinking water containing carboxymethyl cellulose only, treatment-received drinking water containing carboxymethyl celluose, or standard), raw Adiponectin, raw Insulin, raw Leptin, raw IL-6, raw TNF-alpha, calculated Adiponectin (ug/mL), calculated Leptin (pg/mL), calculated Insulin (pg/mL), calculated IL-6 (pg/mL) and calculated TNF-alpha (pg/ml) in plasma from newborn pups.

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