Abstract:

This dataset reports experimental results for Daphnia magna exposed to chemical dispersant Corexit 9500. This dataset includes the results for an acute toxicity test and reproduction test. Number of immobilized neonates, number of neonates, number of males, and number of females are included.

Suggested Citation:

Kohno, Satomi. 2017. Dataset for: Toxic effects of chemical dispersant Corexit 9500 on water flea Daphnia magna. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7J964T5

Publications:

Toyota, K., McNabb, N. A., Spyropoulos, D. D., Iguchi, T., & Kohno, S. (2016). Toxic effects of chemical dispersant Corexit 9500 on water flea Daphnia magna . Journal of Applied Toxicology, 37(2), 201–206. doi:10.1002/jat.3343

Purpose:

To observe effects of dispersant Corexit 9500 on Daphnia magna neonates.

Data Parameters and Units:

TG202_2015.11.27: Daphnia magna reproduction test (OECD TG202) Temperature (deg C), Date (YYYY.MM.DD), Time (hours), Number of individuals, Corexit concentration (ppb) STS_2015.12.5: Daphnia magna reproduction test (OECD TG202) Temperature (deg C), Date (YYYY.MM.DD), Number of male, Number of female, Total number, % of males, % of females, Corexit concentration (ppb), Methyl famesoate concentration (ug/L), Average, Standard deviation, Standard error TG211 1st Brood offspring: Daphnia magna reproduction test (OECD TG211) Type of food, Date (YYYY.MM.DD), Temperature (deg C), Day, Corexit concentration (ppb), Number of reproduction, Period of 1st brood, Number of 1st brood offspring

Methods:

For each experiment below, female neonates (less than 24 hr post hatching) were added to 100 ml beakers with 50 ml of test medium and were exposed to different concentrations of Corexit 9500 including a control. Daphnia magna were maintained at 18 degrees C with a 14 hr light/10 hr dark photoperiod. This dataset includes the results of an acute toxicity text and reproduction test. For the acute toxicity test, 5 Daphnia neonates per beaker were exposed to various Corexit concentrations (0 ppb/control, 0.25 ppb, 0.5 ppb, 1 ppb, 2 ppb, 4 ppb, 8 ppb, 16 ppb, 32 ppb, and 64 ppb). 4 replicates are included for each treatment. The number of immobilized daphnids was counted at 24 hr and 48 hr after the experimental start. For the reproduction test, 1 Daphnia neonate per beaker was exposed to Corexit (0 ppb/control, 0.125 ppb, 0.25 ppb, 0.5 ppb, 1 ppb, 2 ppb, 4 ppb, 8 ppb, 16 ppb, 32 ppb, and 64 ppb). This experiment also had a positive control with 100 ug/l methyl famesoate. 10 replicates are included for each treatment. During the 21 day duration, Daphnia were fed Chlorella vulgaris daily (approx. 20 uL of 4.3 x 108 cells/ml). Neonates were removed daily and number and sex of neonates was recorded.

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