Abstract:

Dioctyl sodium sulfosuccinate or docusate sodium DOSS increases adipogenesis of a murine preadipocyte cell line. 3T3-L1 cells differentiated in the presence of increasing concentrations of DOSS or a standard concentration of Rosi as a positive control. Cells stained with AdipoRed to quantify lipid accumulation and normalized to cell number using Hoechst show dose-dependent accumulation of triacylglycerides. This dataset reports 3T3-L1 cell counts, average of integrated intensity, average of mean intensity, average of integrated intensity background correlation, and average of mean intensity background correlation.

Suggested Citation:

Lexi Temkin, Robert Bowers, Demetri Spyropoulos. 2016. 3t3L1 Cell DOSS Exposure and Adipogenesis. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7X0651K

Publications:

Temkin, A. M., Bowers, R. R., Magaletta, M. E., Holshouser, S., Maggi, A., Ciana, P., … Spyropoulos, D. D. (2015). Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPARγ Activity in Vitro and in Vivo: Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen. Environmental Health Perspectives, 124(1). doi:10.1289/ehp.1409672

Bowers, R. R., Temkin, A. M., Guillette, L. J., Baatz, J. E., & Spyropoulos, D. D. (2016). The commonly used nonionic surfactant Span 80 has RXRα transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers. General and Comparative Endocrinology, 238, 61–68. doi:10.1016/j.ygcen.2016.04.029

Purpose:

Research on adipose tissue with 3T3-L1 cell line derived from (mouse) 3T3 cells that were exposed to DOSS surfactant.

Data Parameters and Units:

docusate sodium DS Protein Expression [personal glucose meter PGM, molecule-imprinted monolith MIM, 10 ppm DOSS, 20 ppm DOSS, 25 ppm DOSS, 50 ppm DOSS, 1microMole Rosi] verses [count, average of integrated intensity, average of mean intensity, average of integrated intensity background correlation, average of mean intensity background correlation]. 3T3-L1 cells treatments: 1: PGM (preadipocyte growth media) 2: MIM (minimal induction media) 3. 10ppm DOSS (dioctyl sodium sulphosuccinate) 4. 20ppm DOSS 5. 25 ppm DOSS, 6. 50 ppm DOSS, 7. 1uM Rosi. 5 replicates treated (A, B, C, D, E).

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