Abstract:

Human Mesenchymal Stem Cells (MSCs), preadipocytes and adipocytes were analyzed for cell specific gene expression. We have confirmed FSTL1 as a reliable preadipocyte marker that is highly expressed in preadipocyte cells and lowly expressed in adipocytes. Similarly, we have identified Leptin as a reliable adipocyte marker since its expression greatly increases in adipocytes compared to preadipocytes. We will use these genes to test crude oil for obesogen potential at the molecular level.

Suggested Citation:

Demetri Spyropoulos, Lexi Temkin, Robert Bowers, John Baatz. 2016. Human Adipocyte/Preadipocyte Gene Expression Assay. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7F18WQ0

Purpose:

To identify genes of the adipogenesis pathway that are differentially expressed in human MSCs, preadipocytes and adipocytes and that will be assessed as markers of obesogenic molecules in Oil/dispersant mixtures.

Data Parameters and Units:

Gene array analysis was used to determine relative expression levels of genes in the adipogenesis pathway. Human_Gene Expression Assay.xlsx: This file includes graphs of relative expression for the different markers Target: adipocyte markers/gene symbol Sample: type of sample (human Mesenchymal Stem Cells (MSCs), human preadipocyte, or human adipocyte) Cq: PCR signal Average: Average of three replicate samples Stdev: standard deviation of the three replicate samples Delta Ct: difference between the sample threshold cycle and the control threshold cycle DeltaDelta to hu_Adipocyte: the difference between Delta Ct for the gene and Delta Ct for the reference Fold Change: 2^DeltaDelta Ct Human_Gene Expression Assay_Adipocytes_graph.pdf Gene expression assay of human MSC, preadipocyte, and adipocyte expression of adipocyte specific genes (normalized to adipocyte gene expression). This graph is included in file Human_Gene Expression.xlsx Human_Gene Expression Assay_DIF.doc Information about the dataset and dataset file list Human_Gene Expression Assay_Preadipocytes_graph.pdf Gene expression assay of human MSC, preadipocyte, and adipocyte expression of preadipocyte specific gene (normalized to adipocyte gene expression). This graph is included in file Human_Gene Expression.xlsx

Methods:

Individual Files: