Abstract:

Biomarkers and PAHs concentrations were measured in caged oysters in May 2010 prior to oil reaching the coastline, and in caged and wild oysters in October 2010 and in 2011 at oiled (Grande Terre) and non-oiled sites (Bay Gardene, Sister Lake, Grand Isle north shore). Hemocyte density, viability, phagocytosis and lysosomal stability were measured in individual oysters. Oysters were then shucked and fragments of gills were used to measure HSP70 stress proteins. Standard tissue sections were processed to assess histopathology. The bulk of the tissue from each oyster was then homogenized and processed to measure Perkinsus marinus infection intensities, calculate condition index and determine PAHs concentrations.

Suggested Citation:

Jerome La Peyre. 2014. Biomarkers and PAHs concentrations in caged and wild oysters, Grande Terre, Breton Sound, Barataria Bay, Sister Lake, 2010-2011.. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7PV6HB6

Purpose:

To assess the impact of the Deepwater Horizon oil spill on Louisiana oysters 2010-2011 as described in: La Peyre JF, Casas S, Miles S. In Press. Oyster responses to the Deepwater Horizon oil spill across coastal Louisiana: Examining oyster health & hydrocarbon bio-accumulation. In Alford B, Peterson MS, Green C (eds), Impacts of oil disasters on marine habitats and fisheries in North America CRC Press.

Data Parameters and Units:

Study 1: Caged oysters sampling ‘pre-coastal oiling’ in May 2010 Study 2: Caged oysters sampling post-oil spill in October 2010 and 2011 Study 3: Wild oysters sampling post-oil spill in October 2010 and August 2011 Sites [Bay Gardene, Sister Lake, Grand Isle, Grande Terre], Hemocyte density (cells per ml), Hemocyte viability (%), Phagocytic ratio (% phagocytic hemocytes, phagocytic index (# zymosan per phagocytic hemocytes), Hemocyte neutral red retention time (min), Gill HSP70 concentration (ng/mg protein), Hsc72 relative content (% standard), Hsp69 relative content (% standard), brown cells density (cells per field of view at 400X), Perkinsus marinus infection intensity (parasites per g wet tissue), condition index, shell height (mm), dry shell weight (g), dry oyster meat weight (g), PAH (ng/g dry tissue weight).

Individual Files: