Abstract:

Zebrafish embryos/larvae are a choice model system for studying early stages of vertebrate development and how these stages can be perturbed by environmental stressors. Petrochemical combustion products elicit developmental toxicity that is associated with transcriptome changes in zebrafish embryos (ZFE). We used microarrays to characterize transcriptome changes in ZFE that had been exposed in the laboratory to oil emulsions collected from the GoM shoreline, ~9 weeks after the Deepwater Horizon blowout. ZFE were exposed to oil emulsions collected (July 1-4, 2010) at the shoreline in Gulfport, MS, Fort Morgan, AL and Perdido Key, FL. ZFE were exposed to emulsions, consisting of varying amounts of alkanes, polynuclear aromatic hydrocarbons (PAHs), sand and seawater that had been “buttered” on the bottom of cups, in which ZFE were suspended in zebrafish medium. ZFE development was monitored by light microscopy. Control ZFE were suspended in zebrafish medium without emulsions. Gross developmental abnormalities, including axial changes, altered swimming patterns and yolk sac and pericardial sac swelling were noted and photographed. ZFE were collected at 48-hr intervals, after exposures had begun, and either placed in clean medium for up to 48 hours more, or, were collected immediately for RNA extraction. and hybridization on Affymetrix zebrafish microarrays, each containing 14,900 probe sets. We extracted RNA from all 30 ZFE in each cup for microarray analysis.

Suggested Citation:

A. Penn, K. Kleinow, F. Xin, A. Bui, R. Xiao, Z. Perveen, P. Polk. 2014. Zebrafish embryo microarrays after 48, 72, and 96 hour exposure intervals to the Deepwater Horizon oil emulsions that reached the GoM shoreline.. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7PZ56SZ

Purpose:

To determine the effect of oil emulsion exposure on zebrafish embryos.

Data Parameters and Units:

Expression profiling by array. Zebrafish exposures to oil emulsions: Control 48 hour (7-18-2010) Control 72 hour (6-16-2010, 6-23-2010, 7-12-2010) Control 96 hour (7-07-2010, 7-25-2010) from Gulfport MS for a 48 hour interval from Gulfport MS for a 72 hour interval from Gulfport MS for a 96 hour interval from Perdido Key FL for a 72 hour interval from Fort Morgan AL for a 72 hour interval

Methods:

Gross developmental abnormalities, including axial changes, altered swimming patterns and yolk sac and pericardial sac swelling were noted and photographed. ZFE/larvae were collected after exposures ended, and placed in clean medium until 192 hr post-fertilization, when larvae were collected for RNA extraction and hybridization on Affymetrix zebrafish microarrays.

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