Abstract:

DNA and RNA were extracted from oil, oil/corexit, and non-oil soils to measure the relative ratios of small nuclear from small mitochondrial material, relative amplification of large nuclear and mitochondrial material, microbial composition between treatments across time (1, 4, 16 weeks) and salinities (4, 12, 18 ppt), apparent lesions on killifish, and the number of lesions on killifish within genetic material from oil and non-oil soil exposures. Sublethal exposures of killifish livers from AIM1 within this same project were preserved in liquid nitrogen. Aim 3: Genotoxicity and soil microbe composition (Mayer, Kuhl, Green).

Suggested Citation:

Chris Green. 2015. Killifish genotoxicity and soil microbe composition from soil treate with oil and Corexit across a range of times and salinities. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78P5XG4

Purpose:

This dataset is represented by long-chain amplicon quantitative PCR and DNA composistion of the mesocosom sediment microbial community and was intended to demonstrate toxicity of oil, Corexit, oil/corexit WAFs at three treatment salinities and throughout time to microbes and examine possible DNA damage in sublethal exposures of Gulf killifish.

Data Parameters and Units:

long and short mitochondrial product, average relative abundance

Individual Files: