Dataset Available

The effects of weather and dispersion on lethal and sublethal toxicity of oil to Gulf killifish across a range of salinities

Authors: Green, Chris
Published On: Mar 21 2014 19:35 UTC
DOI: https://doi.org/10.7266/N7J38QGD
UDI: Y1.x086.000:0002
Files

Individual Files

No. of Downloads: 10

No. of Files: 1

File Size: 600.01 KB

File Format(s):
xlsx

Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
Louisiana State University (LSU)

Research Group:
The Influence of Oil Dispersants on the Biodegradation of South Louisiana Crude Oil and the Resulting Toxicological, Molecular and Physiological Effects on the Gulf Killifish

Point of Contact

Christopher Green
Louisiana State University / Aquaculture Research Station
cgreen@agcenter.lsu.edu

Theme keywords

acute bioassay, salinity, weathering, Gulf killifish

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Abstract:

Aim 1: Characterize the effects of weather and dispersion on lethal and sublethal toxicity of oil across a range of salinities. Thirty mesocosms were established with ~80L of oxic water overlaying ~15L of sediment with an oxic surface layer and an anoxic subsurface similar to conditions seen along the LA coast. Ten mesocosms each were prepared at 4, 12 and 18 ppt. Additional mesocosms were prepared as extra and/or pilot systems. Shading was prepared to equalize the light exposure and temperature between top and bottom row tanks. Following advice from scientists from EPA, it was decided that oil will be pre-weathered prior to addition to mesocosms. This pre-weathering is to mimic oil during a spill event. Volatile components are removed prior to exposure as they would in a spill. During pre-weathering oil (~1:5 oil:water; ~220mL per tank), corexit (~1:50 corexit:water), and oil/corexit mixture was weathered overnight in 4, 12, or 18 ppt water. Water and oil fractions were separated and equally divided into 3 aliquots (1, 4, and 16 week tanks). Aliquots were stored refrigerated in amber glass bottles with Teflon lids until addition to mesocosms. Weathering has been completed at 4 and 12 ppt and 18 ppt.

Suggested Citation:

Green, Chris. 2014. The effects of weather and dispersion on lethal and sublethal toxicity of oil to Gulf killifish across a range of salinities. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7J38QGD

Purpose:

This dataset is represented by acute mortality bioassays and was intended to demonstrate acute toxicity of oil, Corexit, oil/corexit WAFs at three treatment salinities and throughout time.

Data Parameters and Units:

Data parameters and units for this set of files includes daily cumulative mortality, individual age, dilution regimes, and cumulative mortality data at 24, 48, 72, and 96 hours. Acute bioassay data CG.xlsx-- Salinity, Age, Treatment [COREXIT/OIL, Corexit, Dilution, Mesocosm, Oil], Concentration, Percent Alive, Percent Dead [Average, Std.Dev., Frequency], plot of Treatment Dilutions (% v/v) verses Percent Survival.

Individual Files: