Abstract:

Biochemical markers (biomarkers) are induced when fish and other organisms are exposed to PAHs and other hydrocarbons. These biomarkers are indicators of the induction of xenobiotic metabolic pathways and oxidative stress defenses in liver. Ethoxyresorufin-O-dethylase (EROD), a marker of phase I metabolism associated with cytochrome p450-1A, Glutathione-S-transferase (GST) a marker of phase II metabolism, and superoxide dismutase (SOD) a marker of oxidative stress defense were measured in several fish species collected at heavily oiled, lightly oiled, and reference sites during the winter and spring of 2011 after the Deepwater Horizon accident.

Suggested Citation:

Jagoe, Charles et al.. 2014. Inshore fish liver biomarker data, northern Gulf of Mexico, March-May 2011.. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N74B2Z81

Purpose:

To identify and quantify biomarkers of oil exposure in coastal fishes.

Data Parameters and Units:

Location (collection location; Apalachicola NERR, FL, GBNERR, MS, or Bay Jimmy, LA) Date (collection date mo/day/yr) Species (common name, hh catfish = hardhead catfish, spot, croaker, seatrout =spotted seatrout, pinfish, mullet) ID (unique sample identifier code) SOD (units activity per mg protein in s9 fraction) GST (nmols per minute per mg protein in s9 fraction) EROD (pmols per minute per mg protein in s9 fraction. For EROD, activity = 0.00001 indicates enzyme activity was too low to quantify ). Abbreviations: pbd = protein below detection in sample homogenate. SOD overrange = SOD activity above linear range of assay

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