Abstract:

This dataset will include detailed data about the development of a portable Lab-on-CD instrument for in-situ spilled oil detection and its related testing results. Our research targets to develop a highly sensitive and portable instrument that can be used for in-situ detection of spilled oil. The system will have a built-in sample extraction/pre-concentration function to eliminate external sample preparation kit. The oil enrichment is based on Solid Phase Extraction (SPE) and the oil detection is based on fluorescence detection principle. In its heart is a disposable CD-like microfluidic cartridge with a built-in oil pre-concentration unit. All the components on the cartridge will be passive with no power requirement. This micro-cartridge can be replaced easily. The micro-sized optical detection unit will be mounted under the microfluidic detection cartridge. All the active components such as UV light source, photo detector, power supplies, etc., are external to the cartridge and thus, belong to the permanent part of instrument. And the cartridge is supposed to be inexpensively 3D-printed together with the solid phase adsorbent with no assembling requirement. It can therefore be disposable, and eliminates the problem of cleaning and contamination as in a permanently assembled fluidic device. We plan to first design and fabricate all the key components of the prototype system, and build a small dimension and high sensitivity bench-top model to demonstrate feasibility. Then in the next step, we will assemble and design a portable instrument from the tested bench-top components for the implementation of a beta model and start the field tests. Aromatic fractions of crude oil can be stimulated by UV light and emit fluorescent light. Typically, crude oil absorbs light of wavelength between 300 nm and 400nm, and emits light in the range from 450 nm to 650 nm. Thus, the fluorescence detection method will be adopted to determine the existence and concentration of the oil in water. First, the standard testing samples will be applied to test the performance of the instrument we developed and calibrate the instrument. Then, samples collected from the coastal water system will be applied and in-situ test will be conducted. A prototype system, including the centrifugal platform, mechanical microfluidic valves, and the 3d printed CD-like cartridge, has been built and tested. To verify the feasibility, 10ppm (crude oil/water) standard sample, C18, and nonane are applied in the system as the testing sample, solid phase absorbent, and eluant respectively. Testing results show that the fluorescence intensity is significantly enhanced after the SPE process with our prototype system. In this dataset, all the key information during developing the spilled oil detection instrument will be collected and recorded in detail. The related testing results will also be included. The data may contain texts, pictures, charts, videos, etc..