Abstract:

This dataset contains raw CT values for Gulf killifish (Fundulus grandis) larvae (<24h post-hatch) that were exposed to oil and abiotic stressors (hypoxia, high salinity, and high temperature). Larvae were exposed to each condition for 48h, followed by RNA extraction from whole larvae (n= 3 larvae per replicate), cDNA synthesis, and qPCR analysis for a suite of genes. Primer sequences and NCBI accession numbers for each sequence are included as well as primer efficiencies determined through serially diluted standard curves.

Suggested Citation:

Sepulveda, Maria S. and Elizabeth B. Allmon. 2020. qPCR data from oil and environmental conditions in Gulf killifish (Fundulus grandis). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/VBQ60WSC

Publications:

Allmon, E., Serafin, J., Chen, S., Rodgers, M. L., Griffitt, R., Bosker, T., … Sepúlveda, M. S. (2020). Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics. Science of The Total Environment, 142156. doi:10.1016/j.scitotenv.2020.142156

Purpose:

Verification of RNA Seq data through qPCR quantification of a subset of genes.

Data Parameters and Units:

Gene name; sequences, Efficiency; NCBI accession #; Product Length; PAH concentrations (ppb), Dissolved oxygen (ppm), Salinity (ppt), Temperature (degree Celsius); Raw CT Values. PAH concentration: 0, 15 ppb Dissolved Oxygen (DO): 2, 6 ppm Salinity: 3, 30 ppt Temperature: 20, 30 °C

Methods:

RNA extracted from whole larvae (n=3 per replicate) following 48h exposure. cDNA synthesis. qPCR.

Individual Files: