Abstract:

The objective of this project was to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus. C. variegatus from three different life stages (embryonic, ≤ 15 h post-fertilization; post-hatch, ≤ 24 h post-hatch, post-larval ≤ 4 d post-hatch) were exposed to oil, hypoxia, or both for 48 hours. Oil solutions used in exposures were derived from high energy water accommodated fractions (HEWAF) prepared using Macondo well oil following explosion of the Deepwater Horizon oil rig in 2010. Exposure hypoxia was defined as ≤ 2 mg/L of oxygen. All treatments were performed in triplicate. Following exposure, 10 larvae from each replicate were processed for RNA extraction and RNA sequencing.

Suggested Citation:

Simining DJ, Sepulveda MS, Bosker T, DeGuise S, Griffitt RJ. 2019. RNAseq data of developing Cyprinodon variegatus exposed to oil, hypoxia, or both. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-prbm-jv96

Publications:

Rodgers, M. L., Simning, D., Sepúlveda, M. S., De Guise, S., Bosker, T., & Griffitt, R. J. (2020). Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus). Scientific Reports, 10(1). doi:10.1038/s41598-020-58171-8

Purpose:

The purpose of this data set is to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus.

Data Parameters and Units:

Dataset consists of one excel file and contains the following headers- Accession Number, Life Stage, Temperature (°C), Salinity (ppt), Oxic Regime (mg/L), and Oil Concentration (ug/L).

Methods:

HEWAF stock solution was prepared using the method for large volume HEWAF production described by Forth et al. (2017), with modifications. Un-weathered, source oil collected from the DWH riser was used in the preparation of the stock HEWAF solution with a nominal concentration of 1 g/L. HEWAF was prepared using a heavy-duty blender (Waring Commercial). Three liters of artificial seawater were combined with 3.6 mL of source oil in a 3.8 L stainless steel blender container. The water-oil mixture was mixed on the low setting for 30 seconds and transferred to a 4 L separatory funnel. The separatory funnel containing the HEWAF solution was covered with aluminum foil, and allowed to settle for 1 hour. Following the 1 hour settling period, the HEWAF stock solution was used to prepare experiment treatment concentrations by dilution.

Instruments:

The concentration of oil was measured with Waters Quattro Micro GC/MS/MS.

Provenance and Historical References:

Forth, H. P., Mitchelmore, C. L., Morris, J. M. and Lipton, J. (2017), Characterization of oil and water accommodated fractions used to conduct aquatic toxicity testing in support of the Deepwater Horizon oil spill natural resource damage assessment. Environ Toxicol Chem, 36: 1450-1459. doi:10.1002/etc.3672

Individual Files: