Abstract:

Although the toxicity of polycyclic aromatic hydrocarbon (PAHs) in fishes has been well-studied, the mechanisms by which PAHs exert their toxicity are not well understood. Previous work showed that the toxicity of PAHs to fish larvae was enhanced under hypoxia. Therefore, the objective of this work was to use transcriptomics to unravel the molecular changes occurring after co-exposure to both stressors. Gulf of Mexico killifish (Fundulus grandis) larvae (< 24 hours post hatch) were exposed to varying oxygen levels (dissolved oxygen 2, 6 ppm) combined with a low concentration of high energy water accommodated fractions (HEWAF) (∑PAHs 15ppb) for a total of either 24 hours (hypoxia) or 48 hours (normoxia). Transcriptional changes were quantified using RNA-Seq. There were more differentially expressed genes induced by PAH exposure (380) than hypoxia (110). This dataset supports the publications of: Allmon, Elizabeth, Jennifer Serafin, Shuai Chen, Maria L. Rodgers, Robert Griffitt, Thijs Bosker, Sylvain de Guise, Maria S. Sepúlveda. (2020) Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics. Science of the Total Environment 752(15): 142156. https://doi.org/10.1016/j.scitotenv.2020.142156 Jones, Elizabeth R., Danielle Simning, Jenifer Serafin, Maria S. Sepúlveda, Robert J. Griffitt. (2020) Acute exposure to oil induces age and species-specific transcriptional responses in embryo-larval estuarine fish. Environmental Pollution. 263(Pt B):114325. doi:10.1016/j.envpol.2020.114325.

Suggested Citation:

Sepulveda, Maria. 2020. Transcriptome effects of oil and environmental conditions on Gulf of Mexico killifish (Fundulus grandis) larvae. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-sef2-8e60

Publications:

Allmon, E., Serafin, J., Chen, S., Rodgers, M. L., Griffitt, R., Bosker, T., … Sepúlveda, M. S. (2020). Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics. Science of The Total Environment, 142156. doi:10.1016/j.scitotenv.2020.142156

Jones, E. R., Simning, D., Serafin, J., Sepúlveda, M. S., & Griffitt, R. J. (2020). Acute exposure to oil induces age and species-specific transcriptional responses in embryo-larval estuarine fish. Environmental Pollution, 263, 114325. doi:10.1016/j.envpol.2020.114325

Purpose:

To determine the effects of oil in combination with other stressors on the transcriptome of Gulf of Mexico killifish (Fundulus grandis).

Data Parameters and Units:

The headers are- Sample name, title, source name, organism, characteristics: age, characteristics: oil concentration, characteristics: oxygen concentration, molecule, description, processed data file, raw file name, file type, file checksum, file accession number, instrument model (Illumina HiSeq 2500); read length (100 base pair); single or paired-end; file name 1; file name 2; average insert size; standard deviation; gene_id, gene, and locus.

Instruments:

Illumina HiSeq 2500; CASAVA software; Illumina FastQC software; Trimomatic software

Individual Files: