Abstract:

These data are from juvenile red snapper (Lutjanus campechanus) and include gene expression data taken from RNA extracted from livers and spleens, length change over time, weight change over time, and polycyclic aromatic hydrocarbon (PAH) analytes and concentrations at hours 0 and 48. The experiment was run for 17 days at Gulf Coast Research Laboratory under 6 conditions: control, Vibrio control (fish exposed to Vibrio anguillarum only), 0.563 ug/L PAHs, 0.736 ug/L PAHs +Vibrio, 17.084 ug/L PAHs, and 15.779 ug/L PAHs + Vibrio. Fish were sacrificed at days 8, 10, and 17 of the experiment. This dataset supports the publication: Rodgers, M. L., Takeshita, R., & Griffitt, R. J. (2018). Deepwater Horizon oil alone and in conjunction with Vibrio anguillarum exposure modulates immune response and growth in red snapper (Lutjanus campechanus). Aquatic Toxicology, 204, 91–99. doi:10.1016/j.aquatox.2018.09.002

Suggested Citation:

Rodgers, Maria L., Griffitt, Robert J.. 2019. Dataset for: Deepwater Horizon oil alone and in conjunction with Vibrio anguillarum exposure modulates immune response and growth in red snapper (Lutjanus campechanus). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-x4a5-kt65

Publications:

Rodgers, M. L., Takeshita, R., & Griffitt, R. J. (2018). Deepwater Horizon oil alone and in conjunction with Vibrio anguillarum exposure modulates immune response and growth in red snapper (Lutjanus campechanus). Aquatic Toxicology, 204, 91–99. doi:10.1016/j.aquatox.2018.09.002

Purpose:

To understand how biological (length and weight) and immunological (expression of immune genes) parameters in juvenile red snapper (Lutjanus campechanus) are altered when exposed to oil, a bacteria, or both.

Data Parameters and Units:

qPCR data worksheet: Gene expression (relative quantification): gene measured, organ, timepoint (days), treatment (Control, Vibrio Control, 0.563 ug/L Polycyclic Aromatic Hydrocarbons (PAHs), 0.736 ug/L PAHs + Vibrio, 17.084 ug/L PAHs, 15.779 ug/L PAHs + Vibrio). Length and weight data worksheet: Treatment, Tank code, Replicate, Day sacrificed post-start, Start weight (g), End weight (g), Weight difference (g), Start length (mm), End length (mm), Length difference (mm), mm/day length growth, mm/day weight growth. PAHs analytes data worksheet: PAH analyte concentration (ug/L) for different treatments, detection limit.

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