Abstract:

Biodegradation assays with five dibenzopyrene isomers including dibenzo[a,e]pyrene, dibenzo[a,h]pyrene, dibenzo[a,i]pyrene, dibenzo[a,l]pyrene, and dibenzo[e,l]pyrene were conducted in the presence of a consortium of microbes isolated from oil-contaminated sediment. Sediment cores were collected from the Chandeleur Islands in the Gulf of Mexico on May 7, 2018, in subtidal and marsh regions of the western side of the islands where oil from the Deepwater Horizon (DWH) oil spill remains buried. Data includes high throughput sequencing data demonstrating the changes to the individual microbial isolates as a result of the dibenzopyrene contamination.

Suggested Citation:

Melanie J. Beazley; Charles Lewis. 2020. Isomer mixture of dibenzopyrene biodegradation culture assays with microbial isolates from oil-contaminated sediment collected in the Chandeleur Islands, Gulf of Mexico on 2018-05-07. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-hams-bb35

Publications:

Lewis, C. G., & Beazley, M. J. (2020). Impacts of dibenzopyrenes on bacterial community isolated from Gulf of Mexico sediment. MicrobiologyOpen. doi:10.1002/mbo3.1039

Purpose:

The purpose of the study was to determine the effects of five dibenzopyrene isomers on individual microbial isolates in biodegradation culture assays.

Data Parameters and Units:

Accession#, message, sample_name, replicate (1, 2, 3), incubation_day (0, 28), pah (No PAH, Dibenzopyrene mixture), sample collection (Subtidal, Marsh), microcosm_condition (Aerobic, Anaerobic), amplicon (16S-V4, Archaea, ITS, Eukaryote), organism (salt marsh metagenome), host (salt marsh sediment), collection_date (DD-Mon-YY), geo_loc_name (USA: Chandeleur Islands), lat_lon (Latitude and Longitude, 29.895448 N 88.827780 W).

Methods:

A consortium of individual microbes were isolated from Gulf of Mexico sediment contaminated by the Deepwater Horizon oil spill in order to determine if isolates were able to degrade a mixture of dibenzopyrene isomers and how they changed as a result of the high molecular weight polycyclic aromatic hydrocarbon (HMW-PAH) contamination. Culture experiments were conducted in a brackish artificial seawater media (M10b: 4.0 g/L tryptone, 2.5 g/L yeast extract in artificial seawater) amended with (or without) a 1 ppm mixture of the dibenzopyrenes for 28 days. Total DNA of the culture was extracted at the conclusion of the experiment and sequenced by Illumina MiSeq technology.

Instruments:

Illumina MiSeq system.

Individual Files: