Abstract:

Biodegradation assays with dibenzo[a,l]pyrene (DB[a,l]P) were conducted in the presence of a consortium of microbes isolated from oil-contaminated sediment. Sediments were collected from the Gulf of Mexico (29.895448 N 88.827780 W) on 2018-05-07. Data includes high throughput sequencing data demonstrating the changes to the individual microbial isolates as a result of DB[a,l]P contamination.

Suggested Citation:

Melanie J. Beazley, Charles Lewis. 2020. Dibenzo[a,l]pyrene biodegradation culture assays with microbial isolates from oil-contaminated sediment collected from the Gulf of Mexico on 2018-05-07. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-618e-at55

Publications:

Lewis, C. G., & Beazley, M. J. (2020). Impacts of dibenzopyrenes on bacterial community isolated from Gulf of Mexico sediment. MicrobiologyOpen. doi:10.1002/mbo3.1039

Purpose:

The purpose of the study was to determine the effects of dibenzo[a,l]pyrene on individual microbial isolates in biodegradation culture assays.

Data Parameters and Units:

Accession, study (SRP222907), object_status (new), bioproject_accession (PRJNA566417), biosample_accession, library_ID, title (Metagenome of mixed high molecular weight polycyclic aromatic hydrocarbon cultures), library_strategy (AMPLICON), library_source (METAGENOMIC), library_selection (PCR), library_layout (paired), platform (ILLUMINA), instrument_model (Illumina MiSeq), design_description (MiSeq 2x250bp v2), filetype (fastq), filename, and filename2.

Methods:

A consortium of individual microbes were isolated from Gulf of Mexico sediment contaminated by the Deepwater Horizon oil spill in order to determine if isolates were able to degrade DB[a,l]P and how they changed as a result of the high molecular weight polycyclic aromatic hydrocarbon (HMW-PAH) contamination. Culture experiments were conducted in a brackish artificial seawater media (M10b: 4.0 g/L tryptone, 2.5 g/L yeast extract in artificial seawater) amended with (or without) 1 ppm DB[a,l]P for 28 days. Total DNA of the culture was extracted at the conclusion of the experiment and sequenced by Illumina MiSeq technology.

Instruments:

Illumina MiSeq system.

Individual Files: