Abstract:

Blood serum of bottlenose dolphins (Tursiops truncatus) from Barataria Bay, Louisiana, were analyzed for fatty acid profile (FAP) by gas chromatography mass spectrometry (GC/MS).

Suggested Citation:

Napolitano, Michael P. and Maggie Broadwater. 2020. Fatty acid profile data by gas chromatography mass spectrometry for blood serum of the bottlenose dolphin (Tursiops truncatus) from Barataria Bay, Louisiana, 2011– 2018, following the Deepwater Horizon oil spill. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-8q28-za97

Purpose:

In April 2010, the oil drilling platform Deepwater Horizon (DWH) in the northern Gulf of Mexico exploded, which led to a massive oil spill affecting many ecosystems and species, including the bottlenose dolphin (Tursiops truncatus) of Barataria Bay, Louisiana. Dolphins suffered many direct toxic effects of oil exposure and this project sought to determine if any of those effects were observable in their fatty acid profiles from blood serum across six sampling years (2011-2018).

Data Parameters and Units:

Column.header= Description; Sample.name= Unique identifier for sample at fatty acid processing laboratory; Sample.source= Unique identifier for the dolphin from which the sample was collected, or an identifier for laboratory control samples; Sample.collection.date= Date sample was collected in the field (m/d/y); Latitude= Latitude at which sample was collected in the field (decimal degrees); Longitude = Longitude at which sample was collected in the field (decimal degrees); Analysis.batch= Identifier for each group of samples run on the same day; Lab.acquired.date= Date sample was acquired by the laboratory (m/d/y); Data.File.Name File name of each sample; generated by analytical instrument; Vial.number= Unique identifier for the vial in the analytical machine; C10.0= C10:0 – Capric; C12.0= C12:0 – Lauric; C14.0= C14:0 – Myristic; C15.0= C15:0 – Pentadecanoic; C16.0= C16:0 – Palmitic; C17.0= C17:0 – Heptadecanoic; C18.0= C18:0- Stearic; C20.0= C20:0 – Arachidic; C22.0= C22:0 – Behenic; C23.0= C23:0 – Tricosanoic; C24.0; C24:0 – Lignoceric; C25.0= C25:0 – Pentacosanoic; C26.0= C26:0 – Hexacosanoic; C28.0= C28:0 – Octacosanoic; C30.0= C30:0; C10.1= C10:1 – Caproleic; C12.1= C12:1 – Dodecaenoic; C16.1_n9= C16:1(n-9); C17.1= C17:1 – Heptadecaenoic; C18.1_n9= C18:1(n-9) – Oleic; C20.1_n9= C20:1(n-9) – Eicosenoic; C20.3_n9 = C20:3(n-9) – Mead; C22.1_n9= C22:1(n-9) – Erucic; C24.1_n9= C24:1(n-9) – Nervonic; C26.1_n9= C26:1(n-9) – Nervonic; C14.1= C14:1 – Myristoleic; C16.1_n7= C16:1(n-7) – Palmitoleic; C18.1_n7= C18:1(n-7) – Vaccenic; C18.1_n5= C18:1(n-5); C20.3_n7= C20:3(n-7); C14.2= C14:2 – Myristolenic; C16.2= C16:2 – Palmitolenic; C18.2_n6= C18:2(n-6) – Linoleic; C18.2_N6Conj= C18:2(N-6)Conj – Rumenic; C18.3_n6= C18:3(n-6) - Gamma Linolenic; C20.2_n6= C20:2(n-6) – Eicosadienoic; C20.3_n6= C20:3(n-6) - Dihomo-g-linolenic; C20.4_n6= C20:4(n-6) – Arachidonic; C22.2_n6= C22:2(n-6) – Docosadienoic; C22.4_n6= C22:4(n-6) – Adrenic; C22.5_n6= C22:5(n-6) – Docosapentaenoic; C24.2= C24:2; C26.2= C26:2 – Hexacosadienoic; C18.3_n3= C18:3(n-3) - Alpha Linolenic; C20.5_n3= C20:5(n-3) – Eicosapentaenoic; C22.5_n3= C22:5(n-3); C22.6_n3= C22:6(n-3) – Docosahexanoic; Pristanic= Pristanic; Phytanic= Phytanic; C16.1_trans= C16:1 trans; C18.1_trans= C18:1 trans; C18.2_trans= C18:2 trans; 16.0DMA= 16:0DMA; 18.0DMA= 18:0DMA; 18.1DMA= 18:1DMA; Triene.tetraene.ratio= Ratio of C20:3(n-9) to C20:4(n-6); Arachidonic.Docosahexanoic.ratio= Ratio of C20:4(n-6) to C22:6(n-3); Total.saturated.FA= Sum of saturated FAs (C10:0, C12:0, C14:0, C15:0, C16:0, C17:0, C18:0, C20:0, C22:0, C23:0, C24:0, C25:0, C26:0, C28:0, C30:0); Total.w9_n9.FA Sum of n-9 FAs [C10:1, C12:1, C16:1(n-9), C17:1, C18:1(n-9), C20:1(n-9), C20:3(n-9), C22:1(n-9), C24:1(n-9), C26:1(n-9)]; Total.w5.w7= Sum of n-5 and n-7 FAs [C14:1, C16:1(n-7), C18:1(n-7), C18:1(n-5), C20:3(n-7)]; Total.w6_n6.FA; Sum of n-6 FAs [C14:2, C16:2, C18:2(n-6), C18:2(n-6) conj, C18:3(n-6), C20:2(n-6), C20:3(n-6), C20:4(n-6), C22:2(n-6), C22:4(n-6), C22:5(n-6), C24:2, C26:2]; Total.w3_n3.FA= Sum of n-3 FAs [C18:3(n-3), C20:5(n-3), C22:5(n-3), C22:6(n-3)]; Total.branched.FA= Sum of branched FAs (Pristanic acid and phytanic acid); Total.trans.FA= Sum of trans FAs (C16:1 trans, C18:1 trans, C18:2 trans); Total.sat.VLC.FA= Sum of saturated very long chain FAs [C23:0, C24:0, C25:0, C26:0, C28:0, C30:0]; Total.DMA= Sum of dimethyl acetal FAs (16:0 DMA, 18:0 DMA, 18:1 DMA); Total.FA= Sum of all FAs; percent.C10.0; Percent of total FAs represented by C10:0 – Capric; percent.C12.0= Percent of total FAs represented by C12:0 – Lauric; percent.C14.0= Percent of total FAs represented by C14:0 – Myristic; percent.C15.0= Percent of total FAs represented by C15:0 – Pentadecanoic; percent.C16.0= Percent of total FAs represented by C16:0 – Palmitic; percent.C17.0= Percent of total FAs represented by C17:0 – Heptadecanoic; percent.C18.0= Percent of total FAs represented by C18:0- Stearic; percent.C20.0= Percent of total FAs represented by C20:0 – Arachidic; percent.C22.0= Percent of total FAs represented by C22:0 – Behenic; percent.C23.0= Percent of total FAs represented by C23:0 – Tricosanoic; percent.C24.0= Percent of total FAs represented by C24:0 – Lignoceric; percent.C25.0= Percent of total FAs represented by C25:0 – Pentacosanoic; percent.C26.0= Percent of total FAs represented by C26:0 – Hexacosanoic; percent.C28.0= Percent of total FAs represented by C28:0 – Octacosanoic; percent.C30.0= Percent of total FAs represented by C30:0; percent.C10.1= Percent of total FAs represented by C10:1 – Caproleic; percent.C12.1= Percent of total FAs represented by C12:1 – Dodecaenoic; percent.C16.1_n9= Percent of total FAs represented by C16:1(n-9); percent.C17.1= Percent of total FAs represented by C17:1 – Heptadecaenoic; percent.C18.1_n9= Percent of total FAs represented by C18:1(n-9) – Oleic; percent.C20.1_n9= Percent of total FAs represented by C20:1(n-9) – Eicosenoic; percent.C20.3_n9= Percent of total FAs represented by C20:3(n-9) – Mead; percent.C22.1_n9= Percent of total FAs represented by C22:1(n-9) – Erucic; percent.C24.1_n9= Percent of total FAs represented by C24:1(n-9) – Nervonic; percent.C26.1_n9= Percent of total FAs represented by C26:1(n-9) – Nervonic; percent.C14.1= Percent of total FAs represented by C14:1 – Myristoleic; percent.C16.1_n7= Percent of total FAs represented by C16:1(n-7) – Palmitoleic; percent.C18.1_n7= Percent of total FAs represented by C18:1(n-7) – Vaccenic; percent.C18.1_n5= Percent of total FAs represented by C18:1(n-5); percent.C20.3_n7= Percent of total FAs represented by C20:3(n-7); percent.C14.2= Percent of total FAs represented by C14:2 – Myristolenic; percent.C16.2= Percent of total FAs represented by C16:2 – Palmitolenic; percent.C18.2_n6= Percent of total FAs represented by C18:2(n-6) – Linoleic; percent.C18.2_N6Conj= Percent of total FAs represented by C18:2(N-6)Conj – Rumenic; percent.C18.3_n6= Percent of total FAs represented by C18:3(n-6) - Gamma Linolenic; percent.C20.2_n6 = Percent of total FAs represented by C20:2(n-6) – Eicosadienoic; percent.C20.3_n6= Percent of total FAs represented by C20:3(n-6) - Dihomo-g-linolenic; percent.C20.4_n6= Percent of total FAs represented by C20:4(n-6) – Arachidonic; percent.C22.2_n6= Percent of total FAs represented by C22:2(n-6) – Docosadienoic; percent.C22.4_n6= Percent of total FAs represented by C22:4(n-6) – Adrenic; percent.C22.5_n6= Percent of total FAs represented by C22:5(n-6) – Docosapentaenoic; percent.C24.2= Percent of total FAs represented by C24:2; percent.C26.2= Percent of total FAs represented by C26:2 – Hexacosadienoic; percent.C18.3_n3= Percent of total FAs represented by C18:3(n-3) - Alpha Linolenic; percent.C20.5_n3= Percent of total FAs represented by C20:5(n-3) – Eicosapentaenoic; percent.C22.5_n3= Percent of total FAs represented by C22:5(n-3); percent.C22.6_n3= Percent of total FAs represented by C22:6(n-3) – Docosahexanoic; percent.Pristanic= Percent of total FAs represented by Pristanic; percent.Phytanic= Percent of total FAs represented by Phytanic; percent.C16.1_trans= Percent of total FAs represented by C16:1 trans; percent.C18.1_trans= Percent of total FAs represented by C18:1 trans; percent.C18.2_trans= Percent of total FAs represented by C18:2 trans; percent.Total.saturated.FA= Percent of total FAs represented by Sum of saturated FAs (C10:0, C12:0, C14:0, C15:0, C16:0, C17:0, C18:0, C20:0, C22:0, C23:0, C24:0, C25:0, C26:0, C28:0, C30:0); percent.Total.n9.FA= Percent of total FAs represented by Sum of n-9 FAs [C10:1, C12:1, C16:1(n-9), C17:1, C18:1(n-9), C20:1(n-9), C20:3(n-9), C22:1(n-9), C24:1(n-9), C26:1(n-9)]; percent.Total.n5.n7.FA= Percent of total FAs represented by Sum of n-5 and n-7 FAs [C14:1, C16:1(n-7), C18:1(n-7), C18:1(n-5), C20:3(n-7)]; percent.Total.n6.FA= Percent of total FAs represented by Sum of n-6 FAs [C14:2, C16:2, C18:2(n-6), C18:2(n-6) conj, C18:3(n-6), C20:2(n-6), C20:3(n-6), C20:4(n-6), C22:2(n-6), C22:4(n-6), C22:5(n-6), C24:2, C26:2]; percent.Total.n3.FA= Percent of total FAs represented by Sum of n-3 FAs [C18:3(n-3), C20:5(n-3), C22:5(n-3), C22:6(n-3)]; percent.Total.branched.FA= Percent of total FAs represented by Sum of branched FAs (Pristanic acid and phytanic acid); percent.Total.trans.FA= Percent of total FAs represented by Sum of trans FAs (C16:1 trans, C18:1 trans, C18:2 trans)

Methods:

Bottlenose dolphin (Tursiops truncatus) blood was collected during capture-release and stored at −80 °C. To 100 µL of each thawed sample, 100 µL of internal standard (ISTD) solution was added for quantitation of 55 fatty acids. Lipids were first hydrolyzed with HCl at 104 °C for 45 min, the fatty acids were extracted with hexane, and then derivatized to pentafluorobenzyl (PFB) esters with PFB-bromide at room temperature for 15 min. Final solutions were prepared in hexane and then analyzed by gas chromatography mass spectrometry (GC/MS).

Instruments:

gas chromatography mass spectrometry (GC/MS)

Individual Files: