Abstract:

This dataset contains DNA barcoding results of fish eggs collected along the northwestern coast of Cuba and across the Florida Straits from 2017-05-13 to 2017-05-25 using bongo plankton nets. The dataset also includes cruise data from twenty-three different stations collected during R/V Weatherbird II cruise WB1731. Individual eggs were DNA barcoded to identify species of fish spawning in the area. This dataset supports the publication: Kerr, M., Browning, J., Bønnelycke, E.M., Zhang, Y., Hu, C., Armenteros, M., Murawski, S., Peebles, E. and Breitbart, M. (2020). DNA barcoding of fish eggs collected off northwestern Cuba and across the Florida Straits demonstrates egg transport by mesoscale eddies. Fisheries Oceanography, 29(4), 340–348. doi:10.1111/fog.12475

Suggested Citation:

Kerr, Makenzie, Mya Breitbart, Steven Murawski, Ernst Peebles, Maickel Armenteros, Jeremy Browning, Eva-Maria Bønnelycke, Chuanmin Hu, and Yingjun Zhang. 2020. DNA barcoding of fish eggs collected aboard R/V Weatherbird II cruise WB1731 across the Florida Straits and off of northwestern Cuba from 2017-05-13 to 2017-05-25. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/99MT5AFB

Publications:

Kerr, M., Browning, J., Bønnelycke, E., Zhang, Y., Hu, C., Armenteros, M., … Breitbart, M. (2020). DNA barcoding of fish eggs collected off northwestern Cuba and across the Florida Straits demonstrates egg transport by mesoscale eddies. Fisheries Oceanography, 29(4), 340–348. doi:10.1111/fog.12475

Purpose:

Individual eggs were DNA barcoded to identify species of fish spawning in the area.

Data Parameters and Units:

The file "NCBI Data Kerr 2020.xlsx": Accession number, Sequence_name, sample_name, organism isolation_source (seawater), collection_date (M/DD/YYYY), geo_loc_name (Gulf of Mexico, USA), lat_lon (decimal degrees), samp_collect_device (plankton net), and the description. The file "Cruise Data for GRIIDC.xlsx": Date (YYMMDD), Depth (m), Latitude (degree North), Longitude (degree West), Total egg density (eggs/m3). The file "Kerr Supplemental Table.xlsx": Taxon, Common Name, Habitat, Economic Importance, Encounter Frequency (Stations), Station(s) Eggs Encountered. The file "GRIIDC_Cruise_Data_Documentation_Template_Kerr.xlsx" is the cruise documentation for the R/V Weatherbird II cruise WB1731 led by the chief scientist Steven Murawski. Please note that the start date of the cruise is 2017-05-09, not 2015-05-09 and the cruise ID is WB1731, not SB1731 as written in the documentation file.

Methods:

Fish eggs were collected with a plankton net, picked out of plankton sample with microscopes and individually DNA barcoded to identify eggs down to the lowest taxonomic unit. For details, please refer to the associated publication Kerr et al., (2020); https://doi.org/10.1111/fog.12475.

Individual Files: