Abstract:

This dataset contains the lipid class composition of red snapper (Lutjanus campechanus) exposed to a chemically enhanced water-accommodated fraction (CEWAF) of south Louisiana crude oil and a pathogen, obtained using Iatroscan and fatty acid identification. The dataset includes details on experimental setup and conditions, CEWAF preparation, and loading concentrations for pathogen exposures.

Suggested Citation:

Dana Wetzel. 2020. Chemically enhanced water-accommodated fraction (CEWAF) and pathogen exposed Red Snapper: Lipid Class Composition. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/TYJ69KT8

Purpose:

Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in whole body of fish.

Data Parameters and Units:

The dataset contains 1 Excel file with multiple worksheets that detail the experimental setup and conditions (Test Conditions table, Sample Key, System Key), how CEWAF were made (CEWAF Preparation), loading concentrations for pathogen exposures (Vibrio Loads), and lipid class composition. The contents of all the worksheets are described below: Naming convention: Sample IDs are named as "CII-15-0000", where three letter laboratory project code (CII=CIMAGE-II)-two digit year (2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year (0000 through 9999). Please note: MML= Mote Marine Laboratory; MDL= Minimum Detectable Limit; SW = seawater; CEWAF = chemically enhanced water accommodated fraction of oil; R = Reservoir; T = Tank; C/B/C = CEWAF/ bacteria/ CEWAF; B = Bacteria; CEWAF/B/SW = chemically enhanced WAF/bacteria/seawater; CFU = colony-forming unit; ATTC = ATCC is the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines, and other materials. Test Conditions Table: Contains information regarding the setup of the experiments in terms of species, life stage of species, exposure type, number of replicates per treatment, description of treatments, aeration, and exposure design. Sample Key: Field ID, MML, Matrix, Collected Date (MM/DD/YYYY), Collected Time (HH:MM:SS), Species (Red Snapper), Exposure System, Exposure Treatment, comment. System Key: Exposure System Number, Treatment, System Abbreviation. CEWAF Preparation: Date (MM/DD/YYYY), Preparation Time (HH:MM), Oil weight (g), Oil Residual (g), Corexit Volume (ml), Corexit residual (ml), Flask1, Flask2, Flask3, Total (g), Comments. Vibrio Loads: Treatment, Bacteria, Strain, Loading Concentration (CFU/ml), Date (MM/DD/YYYY), Inoculation Time (HH:MM), Treatment, Tank, #fish, CFUs, Start Time and End Time (HH:MM). % Lipid: Whole Body MML, Whole Body % Lipid. Liver TLC: Treatment, Fish ID, Replicate, Liver MML #, % Wax esters & Sterol Esters, %Triglycerides, %Free Fatty Acids, %Cholesterol, %Phospholipids & Polar Lipids. Whole Body TLC: Treatment, Fish ID, Replicate, Liver MML #, % Wax esters & Sterol Esters, %Triglycerides, %Free Fatty Acids, %Cholesterol, %Phospholipids & Polar Lipids.

Methods:

Tissue samples were C-19 fatty acid methyl ester and extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of the sample analyzed with Iatroscan using PeakSimple 444 software. Fatty Acids: 1 uL of the sample analyzed with Agilent 7890A/5975C, Zebron Capillary column.

Instruments:

Iatroscan MK-6s, Agilent 7890A/5975C, Zebron Capillary column.

Error Analysis:

QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.

Individual Files: