Abstract:

This dataset contains oxidative stress, genotoxicity, and immune response biomarkers measured in golden tilefish collected aboard the R/V Weatherbird II cruises WB-1602, WB-1603, WB-1701 and WB-1736 in the Gulf of Mexico from 2015-08-17 to 2017-07-29. The dataset contains the location, date and depth of sample collection. In addition it includes the fish species, fish biometric data (such as weight, sex, liver weight, gonad weight, fork and total length) and concentrations or percentages of biomarkers that indicate oxidative stress (plasma superoxide dismutase, plasma sorbitol dehydrogenase, plasma malondialdehyde), immune response (hematocrit and leukocrit percentages, differential white blood cell counts in whole blood, and plasma lysozyme), and genotoxicity (whole blood micronuclei and nuclear abnormalities). The cruise documentation was provided for the R/V Weatherbird II cruises WB-1602, WB-1603, WB-1701 and WB-1736, led by chief scientists Dr. Steven Murawski and Dr. David Hollander. This dataset supports the publication: Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24

Suggested Citation:

Kristina Deak, Larry Dishaw. 2019. Oxidative stress, genotoxicity, and immune response biomarkers measured in golden tilefish (Lopholatilus chamaeleonticeps) collected aboard multiple R/V Weatherbird II cruises in the Gulf of Mexico from 2015-08-17 to 2017-07-29. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-t3g7-sk92

Publications:

Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24

Purpose:

These data were collected to document Gulf-wide variability in biomarker response in a demersal fish species with historical pollutant exposure. The biomarkers were selected based upon their documented alteration in teleost fishes upon pollutant exposure and their utility in health evaluations.

Data Parameters and Units:

Station_Fish_Year, Species, Station_Year, Station, Fish ID, Year, Health Code, Sex, Standard length (cm), Fork length (cm), Total length (cm), Total weight (kg), Liver weight (kg), Gastrointestinal tract weight (kg), Gonad weight (kg), Month, Day, Latitude (decimal degree), Longitude (decimal degree), Average Depth (m) , Average Temperature (Degrees Celsius), Principal Investigator, Cruise Information, Vessel, Hematocrit (%), Leukocrit (%), Sorbitol dehydrogenase (mIU), Malondialdehyde (uM), Superoxide Dismutase (U/mL), Lysozyme (ug/mL), Micronuclei (#/1000), Binucleated (#/1000), Nuclear buds (#/1000), Blebbed nuclei (#/1000), Notched nuclei (#/1000), Sum Nuclear Abnormalities (#/1000), Lymphocytes (%), Thrombocytes (%), Monocytes (%), Neutrophils (%), and Eosinophils (%).

Methods:

Levels of plasma superoxide dismutase were determined in the laboratory using commercial kits according to manufacturers' protocols (Cayman Chemicals, SOD Assay Kit #706002). Levels of plasma malondialdehyde (via measurement of thiobarbituric acid reactive substances) were determined in the laboratory using commercial kits according to manufacturers' protocols (Cayman Chemicals, TCA Assay Kit #700870). Plasma SDH was measured in the laboratory according to methods developed by Dixon (1987) and modified by others (Shailaja 2003, Webb 2007, Pandelidese 2014). Briefly, NADH was added to samples in a microplate, followed by addition of D-fructose and monitoring the change in absorbance. Micronuclei and nuclear abnormalities were screened and classified manually using oil immersion microscopy (Olympus Binocular Microscope CX23). Differential white blood cell counts were performed manually using oil immersion microscopy (Olympus Binocular Microscope CX23). Hematocrit and leukocrit were determined using a manual hematocrit reader card after spinning capillary tubes in a hematocrit analyzer (HemataStat II Hematocrit Analyzer, EKF Diagnostics). Total plasma lysozyme was determined in the laboratory using a turbidimetric assay developed by Parry (1965) and modified for a 96-well microplate (Grinde 1988, Bado-Nilles 2009, Perrault 2017). Briefly, plasma was exposed to Micrococcus lysodeikticus in a microplate and absorbance was monitored using an ELISA microplate reader (PerkinElmer Enspire, Perkin Elmer, #HH3400000).

Provenance and Historical References:

Bado-Nilles, A., Quentel, C., Thomas-Guyon, H., & Le Floch, S. (2009). Effects of two oils and 16 pure polycyclic aromatic hydrocarbons on plasmatic immune parameters in the European sea bass, Dicentrarchus labrax (Linné). Toxicology in Vitro, 23(2), 235–241. doi:10.1016/j.tiv.2008.12.001 Dixon, D. G., Hodson, P. V., & Kaiser, K. L. E. (1987). Serum sorbitol dehydrogenase activity as an indicator of chemically induced liver damage in rainbow trout. Environmental Toxicology and Chemistry, 6(9), 685–696. doi:10.1002/etc.5620060906 Grinde, B., Jolles, J., & Jolles, P. (1988). Purification and characterization of two lysozymes from rainbow trout (Salmo gairdneri). European Journal of Biochemistry, 173(2), 269–273. doi:10.1111/j.1432-1033.1988.tb13994.x Pandelides, Z., Guchardi, J., & Holdway, D. (2014). Dehydroabietic acid (DHAA) alters metabolic enzyme activity and the effects of 17β-estradiol in rainbow trout (Oncorhynchus mykiss). Ecotoxicology and Environmental Safety, 101, 168–176. doi:10.1016/j.ecoenv.2013.11.027 Parry, R. M., Chandan, R. C., & Shahani, K. M. (1965). A Rapid and Sensitive Assay of Muramidase. Experimental Biology and Medicine, 119(2), 384–386. doi:10.3181/00379727-119-30188 Perrault, J. R., Stacy, N. I., Lehner, A. F., Mott, C. R., Hirsch, S., Gorham, J. C., … Walsh, C. J. (2017). Potential effects of brevetoxins and toxic elements on various health variables in Kemp’s ridley (Lepidochelys kempii) and green (Chelonia mydas) sea turtles after a red tide bloom event. Science of The Total Environment, 605-606, 967–979. doi:10.1016/j.scitotenv.2017.06.149 Shailaja, M. S., & D’Silva, C. (2003). Evaluation of impact of PAH on a tropical fish, Oreochromis mossambicus using multiple biomarkers. Chemosphere, 53(8), 835–841. doi:10.1016/s0045-6535(03)00667-2 Webb, D., & Gagnon, M. M. (2007). Serum Sorbitol Dehydrogenase Activity as an Indicator of Chemically Induced Liver Damage in Black Bream (Acanthopagrus butcheri). Environmental Bioindicators, 2(3), 172–182. doi:10.1080/15555270701591006

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