Abstract:

This dataset contains total polycyclic aromatic hydrocarbon (TPAH) concentrations in exposure seawater solutions and in livers of laboratory-raised Florida pompano exposed to south Louisiana crude oil.

Suggested Citation:

Wetzel, Dana; Sherwood, Tracy; Medvecky, Rebecca; Miller, Christelle. 2019. Total polycyclic aromatic hydrocarbon (TPAH) results: Chemically enhanced water-accommodated fractions (CEWAF)-exposed Florida pompano F1 Generation. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-97xn-0v49

Purpose:

To evaluate the TPAH concentrations of south Louisiana crude oil in exposure seawater and in the tissue of laboratory-raised Florida pompano exposed to south Louisiana crude oil.

Data Parameters and Units:

Sample Key: MML; Field ID; Matrix; Collected Date; Collected Time; Species; Organism Weight(g); Fork Length (cm); Comment Stock CEWAF Preparation: Seawater (L); End Date; End Stir Time; Stir Speed (rpm); Settle Time (hr); Notes; Prepared by CEWAF Dilution Preparation: Date; Time; Carboy ID; Stock Vol (L); Total Stock (L); Final Vol (L); Prepared By Tank Description: Tank; Treatment; Start Date; End Date; Date Spike A; Time Spike A; Time Clean SW A; Date Spike B; Time Spike B; Time Clean SW B; Notes Water TPAH: Field ID; MML; Date; Analytes; MDL (ug/L); ΣPAHs(ug/L) Liver ΣPAH_WW: Field ID; MML; Parent Cross PAH Analytes; MDL (ng/g ww); ΣPAH (ng/g ww) % Lipid: MML; Field ID; % Lipid Note: MML= Mote Marine Laboratory and MDL= Minimum Detectable Limit Nomenclature for sample IDs example: "CII-15-0000" means "Three-letter laboratory project code (CII=CIMAGE-II)-two digit year(2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year(0000 through 9999)".

Methods:

Tissue samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10ml in DCM for analysis. Water samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3510, and concentrated to 1mL in DCM for analysis. PAHs were analyzed using gas chromatography/mass spectrometry in selected ion monitoring mode using a 0.25 um film thickness, 0.25 mm I.D., and 30 m length capillary column. One microliter of the sample was injected at 60ºC, held for 1.5 minutes. The temperature was then increased to 325 ºC at a rate of 8 ºC/ min and held for 3 min.

Instruments:

Dionex ASE300 Accelerated Solvent Extractor, Agilent 7890A/5975C, Zebron Capillary column.

Error Analysis:

Average surrogate recovery 50-150. CCV run before and after every ten samples. CCV passing recovery must be average less than 20%. CCV means Continued Calibration Verification.

Individual Files: