GRIIDC | R6.x805.000:0069

Dataset Available

Chemically enhanced water-accommodated fractions (CEWAF)- exposed Florida pompano reproduction: Thyroid Function Assays

Authors: Dana Wetzel

Published On: Apr 07 2020 10:24 UTC

DOI: https://doi.org/10.7266/n7-3nty-7169

UDI: R6.x805.000:0069

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Individual Files

No. of Downloads: 1

No. of Files: 1

File Size: 47.43 KB

File Format(s):
xlsx

Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-VI

Research Group:
Center for the Integrated Modeling and Analysis of Gulf Ecosystems III (C-IMAGE III)

Point of Contact

Dana Wetzel
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
dana@mote.org

Theme keywords

Thyroid, Reproduction, Tri-iodothyronine (T3), Thyroxine (T4), Thyroid Stimulating Hormone (TSH), Florida Pompano, Trachinotus carolinus, Chemically enhanced water accommodated fraction (CEWAF), Crude oil

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Abstract:

This dataset contains concentrations of Tri-iodothyronine (T3 ), Thyroxine (T4), and Thyroid Stimulating Hormone (TSH) in adult pompano after exposure to a chemically enhanced water-accommodated fraction (CEWAF) of south Louisiana crude oil and reproduction.

Suggested Citation:

Dana Wetzel. 2020. Chemically enhanced water-accommodated fractions (CEWAF)- exposed Florida pompano reproduction: Thyroid Function Assays. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-3nty-7169

Purpose:

Identify changes in thyroid function in adult pompano after exposure to CEWAF of south Louisiana crude oil and reproduction.

Data Parameters and Units:

Dataset consists of 1 Excel file with multiple worksheets that detail the experimental setup and conditions (Naming Convention, T208 Sample Key, Test Conditions Table, Tank Descriptions, and ELISA). The worksheet "Naming Convention": Sample IDs are named as "CII-15-0000", where three letter laboratory project code (CII=CIMAGE-II)-two digit year (2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year(0000 through 9999). The worksheet “T208 Sample Key” includes - Collected Date (MM/DD/YYYY), Field ID (Fish #), Matrix (Plasma-EDTA), MML Number, Species (Florida Pompano), Gender (Male/Female), Weight (g), and Length (cm). The worksheet “Tank Descriptions” includes - Tank, # Female, # Male, Treatment (Control/CEWAF), Start Date (MM/DD/YYYY), End Date (MM/DD/YYYY), Date Spike A, Time Spike A, Date Spike B, Time Spike B, and Notes. The worksheets “ELISA” includes - Spawning tank, Goiter, Treatment (CEWAF/Control), Fish ID, and replicate measurements of Cusabio T4 (ng/ml), Cusabio TSH (Ug/ml), and Cusabio T3 (ng/ml). The worksheet “Test Conditions Table” includes - Test Name (Pompano Adult Water Exposure), Test ID: 208, Species tested (Florida Pompano), Life-stage/age (Adult), Test Duration, Exposure Type, Renewal frequency, Number of replicates per treatment (9), Organisms per tank, Temperature, Salinity, Number of Treatments, and the description of treatments.

Methods: