Abstract:

This dataset contains a histological examination of livers of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard the R/V Weatherbird II cruises WB-1304, WB-1403, WB-1502, WB-1602, WB-1603, and WB-1701 in the Gulf of Mexico from 2012-08-14 to 2016-09-06. The dataset includes the identified presence of histological changes and images of the gross and histological evaluations. Formalin-fixed tissues were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from the sample jar. A second photo was taken of the samples to highlight gross morphology. If an abnormality was observed, additional photos were procured of the abnormality. Histological sections were stained with routine Hematoxylin and Eosin. Slides were evaluated blind in respect to the location and date of collection. After a preliminary evaluation, several fish were chosen to characterize specific lesions with special stains. Special stains included Periodic Acid-Schiff (PAS) with and without diastase, Gram stain, Perl’s Iron, Von Kossa, and Masson’s trichrome. Microscopic examination was performed using a Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan) and photomicrographs were taken with an Accu-scope Excelis HD (Commack, NY). The cruise documentation was provided for the R/V Weatherbird II cruises WB-1304, WB-1403, WB-1502, WB-1602, WB-1603, and WB-1701 led by chief scientists Dr. Steven Murawski and Dr. David Hollander. This dataset supports the publication: Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24

Suggested Citation:

Snyder, S.M.; Fogelson, S.; Murawski, S.A.; E.L. Pulster. 2019. Histological examination of livers of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard multiple R/V Weatherbird II cruises in the Gulf of Mexico from 2012-08-14 to 2016-09-06. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-460y-rz32

Publications:

Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24

Purpose:

Examine normal golden tilefish liver anatomy and the prevalence and frequency of microscopic hepatocellular changes.

Data Parameters and Units:

The file "GRIIDC Snyder Golden Tilefish Pathology Report1 0066.xlsx" contains the following: Organization leading the cruises Research vessel used for the cruises P.I. of the project Month of sample collection Date of sample collection Year of sample collection Station where fish was caught Latitude and longitude of the station where fish was caught in decimal degrees Fish # Full fish ID (station + fish #) Species samples Tissue analyzed Accession number assigned by Fish Head Labs for blind evaluation of samples Gross evaluation description Histological evaluation description Legend for identifying presence of abnormality (present = 1, absent = 0) Legend for identifying severity of certain abnormalities (scale 0-6 where 0 = none, 6 = severe) Lipid-type vacuolar change Lipid-type vacuolar change severity Glycogen-type vacuolar change Glycogen-type vacuolar change severity Hepatocellular adenoma Basophilic focus Pale focus Atrophy Parasite (found outside of granuloma) Granuloma(s) Pigmented macrophage aggregates Pigmented macrophage aggregate severity Bile duct fibrosis Bile duct fibrosis severity Bile duct duplication Bile duct fibrosis and duplication severity Necrosis Inflammation, lymphocytic aggregation Cyst Ito cell hyperplasia The folders "Gross Images" and "Histo Images" contains the images of the samples for gross evaluation and histological evaluation.

Methods:

Formalin-fixed tissues were submitted by the University of South Florida College of Marine Sciences, Population and Marine Ecosystem Laboratory to Fishhead Labs, LLC for gross examination and histological evaluation. Tissues from each sample container were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from the sample jar. A second photo was taken of the samples to highlight gross morphology. If an abnormality was observed, additional photos were procured of the abnormality. Histological sections were stained with routine Hematoxylin and Eosin. Slides were evaluated blind in respect to the location and date of collection. After a preliminary evaluation, several fish were chosen to characterize specific lesions with special stains. Special stains included Periodic Acid-Schiff (PAS) with and without diastase, Gram stain, Perl’s Iron, Von Kossa, and Masson’s trichrome. Microscopic examination was performed using a Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan) and photomicrographs were taken with an Accu-scope Excelis HD (Commack, NY).

Instruments:

Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan), Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan), Accu-scope Excelis HD (Commack, NY).

Individual Files: