Abstract:

Sediment samples collected from the northern Gulf of Mexico were used for incubations in which microbial respiration rates were measured as a proxy for biodegradation of hydrocarbons. Following the incubation, microbial communities were characterized from incubation samples using next generation sequencing of SSU rRNA genes. A systematic approach was used whereby pressure, temperature, and hydrocarbon substrate were varied.

Suggested Citation:

Sun, Xiaoxu. 2020. Impacts of high pressure on hydrocarbon biodegradation. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-eg4t-1g22

Purpose:

The purpose of the dataset was to compare the impact of hydrostatic pressure on biodegradation of crude oil.

Data Parameters and Units:

Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 09/08/2016; Decimal latitude and longitude: 28 58.946N 87 53.665W; Depth of water in meters: 1468; Depth of sediment in centimeters 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences; Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 09/09/2016; Decimal latitude and longitude: 29 07.444N -87 15.94W; Depth of water in meters: 924; Depth of sediment in centimeters: 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences; Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 08/11/2016; Decimal latitude and longitude: 18 59.149 92 09.080; Depth of water in meters:19; Depth of sediment in centimeters 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences

Methods:

The samples were collected using multicore. The retrieved samples were extruded based on depth on board. Samples were kept at 4 degrees Celsius until further processing. The preparation of samples for incubations were conducted in a cold room at 4C the whole time for maintaining the temperature. Surface sediment samples were mixed with filtered seawater from the same site. 1ul crude oil was provided. Samples were incubated in heat-sealable polypropylene tubes in pressure-maintaining cells. Oxygen was measured as a proxy for oil degradation, and no oil control/ sterile controls were prepared as the background. DNA was extracted at different time points and sequenced for the SSU rRNA on an Illumina MiSeq.

Instruments:

Illumina MiSeq

Individual Files: