Abstract:

This dataset contains pathological findings in red snapper (Lutjanus campechanus) livers collected aboard the multiple Weatherbird II cruises WB1304 (2012-08-13 to 2012-08-25), WB1403 (2013-08-15 to 2013-08-30), WB1502 (2014-08-05 to 2014-08-20), WB1602 (2015-08-15 to 2015-08-30), WB1736 (2017-07-18 to 2017-08-01) in the north central region of the Gulf of Mexico, post-Deepwater Horizon. The dataset includes the identified presence of histological changes and images of the gross and histological evaluations. Formalin-fixed tissues were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from the sample jar. A second photo was taken of the samples to highlight gross morphology. After a preliminary evaluation, several fish were chosen to characterize specific lesions with special stains. Special stains included Periodic Acid-Schiff (PAS) with and without diastase, Gram stain, Perl’s Iron, Von Kossa, and Masson’s trichrome. Microscopic examination was performed using a Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan) and photomicrographs were taken with an Accu-scope Excelis HD (Commack, NY). The cruise documentation was provided for the Weatherbird II cruises WB1304, WB1403, WB1502, WB1602, and WB1736 led by chief scientists Dr. Steven Murawski and Dr. David Hollander.

Suggested Citation:

Pulster, E.L.; Fogelson, S., Murawski, S.A.. 2019. Pathological findings in red snapper livers collected aboard multiple R/V Weatherbird II cruises in the north central region, Gulf of Mexico from 2012-08-13 to 2017-08-01. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-nmsy-tq94

Publications:

Pulster, E. L., Gracia, A., Snyder, S. M., Romero, I. C., Carr, B., Toro-Farmer, G., & Murawski, S. A. (2019). Polycyclic Aromatic Hydrocarbon Baselines in Gulf of Mexico Fishes. Scenarios and Responses to Future Deep Oil Spills, 253–271. doi:10.1007/978-3-030-12963-7_15

Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24

Purpose:

Data were collected as part of the C-IMAGE Gulf-wide surveys in order to characterize the impacts of the Deepwater Horizon oil spill on benthic species.

Data Parameters and Units:

The file "GRIIDC Pulster Red Snapper Pathology Report 2017.0508.xlsx": Contains cruise name, Vessel name, Principal Investigator, Year of sample collection (YYYY), Station ID, Fish #, Fish ID, Longitude (decimal degrees), Latitude (decimal degrees), Species (Red Snapper), Tissue (Liver), Lesion, Fishhead labs accession numbers F17-172, Gross evaluation, Histological evaluation. The identified presence of histological changes includes - Lipid-type vacuolar change Present, Lipid-type vacuolar change Severity Score, Glycogen-type vacuolar change Present, Glycogen-type vacuolar change severity score, Hepatocyte loss with radiating material (Artifact) Present, Hepatocyte loss with radiating material (Artifact) Severity Score, Peripancreatic Macrophage Aggregates Present, Peripancreatic Macrophage Aggregates Severity Score, Pigmented Macrophage Aggregate Present, Pigmented Macrophage Aggregate Severity Score, Fibrosis Present, Fibrosis Severity Score, Hemosiderin/Liposucin/ ceroid Present, Edema Present, Parasites / Nematodes Present, Lymphocytic Inflammation Present, Lymphoplasmatic Inflammation Present, Bacteria Present, Single Cell Necrosis Present, Single Cell Necrosis Severity Score, Bile Duct Necrosis Present, Bile Duct Hyperplasia Present, Bile Duct Hyperplasia Severity Score, Bile Duct Autolysis Present, Hyperplastic Nodule Present, Abnormal Basophilic Focus Present, Atrophy Present, Myxozoan Plasmodia Present, and Granulomas Present. Presence Legend: Score 0 means not present in the tissue. Score 1 means present in the tissue. The severity of vacuolar changes: Score 0 means no change. Score 1 means minimal change. Score 2 means mild change. Score 3 means mild to moderate change. Score 4 means moderate change. Score 5 means moderate to severe change. Score 6 means severe change. The folders "Red Snapper Gross Evaluation Images 2017" and "Red Snapper Histological Evaluation Images 2017" contains the images of the samples for gross evaluation and histological evaluation.

Methods:

Formalin-fixed tissues were submitted by the University of South Florida College of Marine Sciences, Population and Marine Ecosystem Laboratory to Fishhead Labs, LLC for gross examination and histological evaluation. Tissues from each sample container were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from the sample jar. A second photo was taken of the samples to highlight gross morphology. If an abnormality was observed, additional photos were procured of the abnormality. Histological sections were stained with routine Hematoxylin and Eosin. Slides were evaluated blind with respect to the location and date of collection. After a preliminary evaluation, several fish were chosen to characterize specific lesions with special stains. Special stains included Periodic Acid-Schiff (PAS) with and without diastase, Gram stain, Perl’s Iron, Von Kossa, and Masson’s trichrome. Microscopic examination was performed using a Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan) and photomicrographs were taken with an Accu-scope Excelis HD (Commack, NY).

Individual Files: