Data Parameters and Units:
The file contains multiple worksheets that detail the experimental setup and conditions (Test Conditions table, Sample Key, System Key), how stock and diluted CEWAF were made (Stock CEWAF Preparation, CEWAF Dilution Preparation respectively), loading concentrations for pathogen exposures (Vibrio Loads), and TPAH concentrations (TPAH_water, TPAH_Livers, TPAH_Whole Bodies).
Naming convention: Sample IDs are named as "CII-15-0000", where three letter laboratory project code (CII=CIMAGE-II)-two digit year (2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year(0000 through 9999). Please note: MML= Mote Marine Laboratory; MDL= Minimum Detectable Limit; SW = seawater; CEWAF = chemically enhanced water accommodated fraction of oil; R = Reservoir; T = Tank; C/B/C = CEWAF/ bacteria/ CEWAF; B = Bacteria; CEWAF/B/SW = chemically enhanced WAF/bacteria/seawater; CFU = colony-forming unit; ATTC = ATCC is the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines, and other materials.
Test Conditions Table: Contains information regarding the setup of the experiments in terms of species, life stage of species, exposure type, number of replicates per treatment, description of treatments, aeration, and exposure design.
Sample Key: Field ID, MML, Matrix, Collected Date (MM/DD/YYYY), Collected Time (HH:MM:SS), Species, Exposure System, Exposure Treatment, Comment.
System Key: Exposure System, Treatment.
CEWAF Preparation: Date (MM/DD/YYYY), Preparation Time (HH:MM), Oil weight (g), Oil Residual (g), Corexit Volume (ml), Corexit residual (ml), comments.
Vibrio Loads: Treatment, Bacteria, Strain, Loading Concentration (CFU/ml), Date (MM/DD/YYYY), Inoculation Time (HH:MM), Treatment, Tank, #fish, CFUs, Start Time and End Time (HH:MM).
TPAH_water: MML, System, Field ID, Date (MM/DD/YYYY), Day, Analyte, MDL (ug/L), ΣPAHs Raw and Adjusted for MDL (ug/L).
TPAH_Livers: MML, System, Field ID, Date (MM/DD/YYYY), Analyte, MDL (ng/g), ΣPAHs Raw and Adjusted for MDL (ng/g).
TPAH_Whole Bodies: MML, System, Field ID, Date (MM/DD/YYYY), Analyte, MDL (ng/g), ΣPAHs Raw and Adjusted for MDL (ng/g).
% Lipid: System, Field ID, Liver MML, Liver % Lipid, Whole Body MML, Whole Body % Lipid.
Methods:
Tissue samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10ml in DCM for analysis. Water samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3510, and concentrated to 1mL in DCM for analysis. PAHs were analyzed using gas chromatography/mass spectrometry in selected ion monitoring mode using a 0.25 um film thickness, 0.25 mm I.D., and 30 m length capillary column. One microliter of sample was injected at 60ºC, held for 1.5 minute. Temperature was then increased to 325 ºC at a rate of 8 ºC/ min, and held for 3 min.
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