Abstract:

Adult zebrafish (Danio rerio) were separated into 4 experimental groups, 1) Normoxia and control food, 2) Normoxia and crude oiled food, 3) Hypoxia and control food and 4) Hypoxia and crude oiled food and exposed for 3 weeks. Body mass was estimated every week and at the third week maximum heart rate was estimated by impedance under drug injection protocol (atropine, isoproterenol and propanolol). After exposure the experimental groups were reproduced, and the offspring were challenged to survive in control water or oil-contaminated water. Survival and heart rate of the offspring were analyzed on a daily basis over a 5-day period. Global DNA methylation was analyzed by colourometry.

Suggested Citation:

Bautista, Naim Bautista and Warren Burggren. 2021. Transgenerational effects of the combined exposure to crude oil and hypoxia in the zebrafish (Danio rerio). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/RG4R1PMR

Purpose:

To determine the effects of parental exposure to environmental stressors on offspring performance in zebrafish (Danio rerio).

Data Parameters and Units:

Headers include Group (1=control; 2= hypoxia; 3= HEWAF; 4= HH), sex (male/female), day (#), before atropine, fH(atrophine), fH(isoproterenol), length (mm), Measurements of body mass (mg), ventricle mass (mg), gonad mass (mg), tissue (gonads, heart), Condition factor (K), survival (%), heart rate (beats per minute), global DNA methylation (%), hypoxia resistance (seconds). Dataset also contains a readme text file “Read_me_Spreadsheets” containing the explanation/specifications for each spreadsheet and the content in each of its columns. Empty cells demark that it was not possible to determine the specific value for that specific variable. Another readme text titled “Read_me_video_files_specifications” contains the specifications for the video files and to which experimental group they belong.

Methods:

High Energy Water Accommodated Fractions of crude oil (HEWAF) were prepared following standard protocols (Bautista et al., 2019; Forth et al., 2017; Mager et al., 2014; Reddam et al., 2017). Briefly, 2000 mg of Source Oil B acquired from the Deepwater Horizon oil spill were added into 1L of conditioned aquarium water (60mg/L of instant ocean salts buffered at pH 7.6) and blended for 30 seconds in a commercial blender (WaringTM CB15). The mixture was allowed to settle for 1 h into a separation funnel, after which 100 ml were drawn out through the bottom port of the funnel and discarded. 600ml of the remaining solution was considered to be 100% HEWAF and was used to prepare the oiled-diet used during this experiment.

Instruments:

WaringTM CB15

Provenance and Historical References:

Bautista, Naim M., Tanushri Pothini, Kelly Meng, and Warren W. Burggren. (2019). Behavioral consequences of dietary exposure to crude oil extracts in the Siamese fighting fish (Betta splendens). Aquatic Toxicology 207, 34-42. doi:10.1016/j.aquatox.2018.11.025 Forth, Heather P., Carys L. Mitchelmore, Jeffery M. Morris, and Joshua Lipton. (2017). Characterization of oil and water accommodated fractions used to conduct aquatic toxicity testing in support of the Deepwater Horizon oil spill natural resource damage assessment. Environmental Toxicology and Chemistry 36(6), 1450-1459. doi:10.1002/etc.3672 Mager, Edward M., Andrew J. Esbaugh, John D. Stieglitz, Ronald Hoenig, Charlotte Bodinier, John P. Incardona, Nathaniel L. Scholz, Daniel D. Benetti, and Martin Grosell. (2014). Acute embryonic or juvenile exposure to deepwater horizon crude oil impairs the swimming performance of mahi-mahi (Coryphaena hippurus). Environmental Science & Technology 48(12), 7053-7061. doi:10.1021/es501628k Reddam, Aalekhya, Edward M. Mager, Martin Grosell, and M. Danielle McDonald. (2017). The impact of acute PAH exposure on the toadfish glucocorticoid stress response. Aquatic Toxicology 192, 89-96. doi:10.1016/j.aquatox.2017.08.014 Incardona, John P., Tanya L. Swarts, Richard C. Edmunds, Tiffany L. Linbo, Allisan Aquilina‐Beck, Catherine A. Sloan, Luke D. Gardner, Barbara A. Block, and Nathaniel L. Scholz. (2013). Exxon Valdez to Deepwater Horizon: Comparable toxicity of both crude oils to fish early life stages. Aquatic Toxicology 142‐143:303–316. doi:10.1016/j.aquatox.2013.08.011

Individual Files: