Abstract:

This dataset contains measurements of sarcomere shortening and other biophysical aspects of contractile function in mahi-mahi (Coryphaena hippurus) heart cells following exposure to varied adrenaline concentrations ranging from 0.001 micromolar to 10 micromolar.

Suggested Citation:

Heuer, R., Stieglitz, J., Benetti, D., Grosell, M., Crossley, D.. 2020. Effects of adrenaline on mahi-mahi (Coryphaena hippurus) heart cells. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-d4am-d715

Purpose:

To determine if adrenaline protects myocytes against polycyclic aromatic hydrocarbon (PAH) toxicity.

Data Parameters and Units:

Excel file: “Effects of adrenaline on mahi heart cells” contains all raw data including biometrics of mahi that were used for cellular isolations. For raw data, in each experiment (Cells 1-18) there is a control and an exposure ranging from 0.001 -10 micromolar. For biometric data, it includes - Cell number, heart mass (grams), body mass (kilograms), and fork length (centimeters). Please note that - no data means that the software fitting that particular endpoint resulted in an error and could not provide a data point, and hence the measurement was not taken. Text file: “Effects of adrenaline on mahi heart cells readme” is a ReadMe file and contains additional information including methods.

Methods:

Mahi hearts were extracted and retrograde perfused with a solution including proteolytic enzymes (Trypsin Type 1X 0.5 milligrams per milliliter), Collagenase Type 1A (0.37-0.43 milligrams per milliliter), and fatty-acid free bovine serum albumin (0.75 milligrams per milliliter). Sarcomere length and other kinetic properties of mahi cardiomyocyte shortening were recorded in real-time using an IonOptix Myocyte Contractility system comprised of a perfusion chamber (FHDRCC1, IonOptix) mounted to an inverted microscope (Motic AE31) attached to a specialized fast digitizing dimensioning camera (MyoCam-S, IonOptix). The perfusion chamber was instrumented with electrodes connected to a MyoPacer stimulator (IonOptix) for field stimulation to induce myocyte shortening. The average sarcomere length was determined using SarcLens within IonWizard 6.0. IonOptix software was used to analyze sarcomere shortening (our index of cell contractility) in addition to the other kinetic properties of mahi cardiomyocyte shortening including departure velocity, return velocity, time to peak contraction, time to recovery, time at a maximal rate of departure, and time at a maximal rate of return. To obtain an individual cell recording, a small set of cardiomyocytes were gently transferred to the perfusion chamber and allowed to settle on the bottom for five to ten minutes prior to perfusion with control saline. Then, a randomly selected healthy cell was chosen for an individual cell recording. Cardiomyocytes were stimulated with the IonOptix system at a frequency of 0.5 Hz and perfused with control saline followed by control saline containing a dilution of adrenaline (10 micromolar, 1 micromolar, 0.1 micromolar, 0.01 micromolar, or 0.001 micromolar). Recorded endpoints during this process can be found in the Excel file “Effects of adrenaline on mahi heart cells."

Individual Files: