Abstract:

This dataset contains alkane degradation and total petroleum hydrocarbon (TPH) data for bacteria exposed to different treatments. Pseudomonas putida, Bacillus pumilus, Pseudomonas putida, and Pseudomonas fulva were collected from Bay Jimmy, Louisiana, November-December, 2013. Bacteria were seeded at a concentration of 10^7 cells/mL in 25 mL of mineral salt medium with 1% crude oil in bacteria treatments. This dataset supports the publication: Zheng, M., Wang, W., Hayes, M., Nydell, A., Tarr, M. A., Van Bael, S. A., & Papadopoulos, K. (2017). Degradation of Macondo 252 oil by endophytic Pseudomonas putida. Journal of Environmental Chemical Engineering, 6(1): 643-648. doi:10.1016/j.jece.2017.12.071

Suggested Citation:

Mengyuan Zheng. 2018. Dataset for: Degradation of Macondo 252 oil by endophytic Pseudomonas putida. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7B856MQ

Publications:

Zheng, M., Wang, W., Hayes, M., Nydell, A., Tarr, M. A., Van Bael, S. A., & Papadopoulos, K. (2017). Degradation of Macondo 252 oil by endophytic Pseudomonas putida. Journal of Environmental Chemical Engineering. doi:10.1016/j.jece.2017.12.071

Purpose:

To measure oil degradation by bacteria.

Data Parameters and Units:

Biosurfactant (mg/L), TPH degradation (%), Alkane degradation (%), cell concentration (number/mL), petroleum hydrocarbons C14 to C23 (peak area), internal standard (peak area) and total petroleum hydrocarbon (sum of all peaks area), treatment (A= Pseudomonas putida Strain SV699 ; _ A+B and A,B = _P. putida Strain SV699 and Bacillus pumilus Strain SV739; A+C and A,C = P. putida Strain SV699 and __ P. putida Strain SV820; A+B+C+D and A,B,C,D = P. putida Strain SV699 and B. pumilus Strain SV739 and __ P. putida Strain SV820 and Pseudomonas fulva Strain SV807; control (1% crude oil incubated with Mineral Salt Medium), time (day 0, day 25)

Methods:

This dataset reports experimental results for 4 individual replicates from each of 5 treatments: A= Pseudomonas putida Strain SV699 ; _A,B = _P. putida Strain SV699 and B. pumilus Strain SV739; A,C = P. putida Strain SV699 and __ P. putida Strain SV820; A,B,C,D = P. putida Strain SV699 and B. pumilus Strain SV739 and __ P. putida Strain SV820 and Pseudomonas fulva Strain SV807; control (1% crude oil incubated with Mineral Salt Medium). Bacteria were seeded at a concentration of 10^7 cells/mL in 25 mL of mineral salt medium with 1% crude oil in bacteria treatments. Total petroleum hydrocarbons were measured via GC/FID using EPA method 8015 after 25 days. Biosurfactant production was measured via an oil spreading assay after 25 days.

Individual Files: