Abstract:

The dataset consists of: a. z-stack images (.tiff) of 20 um and 50 um drop diameters at different time intervals for motile bacteria; b. z-stack images (.tiff) of 20 um and 50 um drop diameters at different time intervals for non-motile bacteria; c. z-stack images (.tiff) 20 um and 50 um drop diameters at different surfactant concentrations for motile bacteria; d. z-stack images (.tiff) 20 um and 50 um drop diameters at different surfactant concentrations for non-motile bacteria The data analyzed consists of: a. number of cell and cells normalized with drop surface area as a function of time; b. cell areal density as a function of surfactant concentration; c. interfacial tension as a function of surfactant concentration; c. cell areal density as a function of interfacial tension. This project investigated the effect of bacteria motility and surfactant concentration on the initial adhesion of Marinobacter hydrocarbonoclasticus and Halomonas titnicae at the interface of hexadecane droplets suspended in seawater. This dataset supports the publication of Dewangan, Narendra K. and Conrad, Jacinta C. “Bacterial motility enhances adhesion to oil droplets.” Soft Matter (2020). https://doi.org/10.1039/D0SM00944J

Suggested Citation:

Dewangan, Narendra K. and Jacinta C. Conrad. 2020. Dataset for: Bacterial motility enhances adhesion to oil droplets. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/4VJXD55E

Publications:

Dewangan, N. K., & Conrad, J. C. (2020). Bacterial Motility Enhances Adhesion to Oil Droplets. Soft Matter. doi:10.1039/d0sm00944j

Purpose:

This project investigated the effect of bacteria motility on bacteria adhesion to oil droplets. Adhesion of cells on oil droplets were analyzed over time, and as a function of time for motile and non-motile bacteria.

Data Parameters and Units:

Dataset consists of folders of images and videos, 3 Excel files and a readme Word document. Details on the acquisition of data all images and videos are described in the Excel file called “Metadata”. The other 2 Excel files are used to present data on Zeta potential and the response of the stain “H. titanicae 10BA” to carbonyl cyanide m-chlorophenyl hydrazine (CCCP). The headers are: Absorbance (a.u.), Interfacial tension (IFT, mN/m), Concentration (ppm and g/l), Surface energy (mN/m), Time (min), Drop diameter (um), Cell areal density (um^-2), Number of cells (unitless), EG (ethylene glycol), DIM (diiodomethane), L (left contact angle in degrees of sessile drop), R (right contact angle in degrees of sessile drop), SE (surface energy in mN/m), micro-cm/V-s), Zeta potential (mV)

Methods:

Z-stack images of cells adhering to oil droplets were acquired using confocal system VTinfinity (Visitech, Sunderland, UK) on an inverted microscope (Leica DMI4000) with a 40X objective lens (HCX PL APO, NA 1.25-0.75). Surface energy and contact angle measurement was done using a goniometer (Dataphysics OCA 15EC). Zeta potential of cell suspensions and emulsions were measured using Nicomp 380 zeta potential analyzer.

Instruments:

Leica DMI4000; Dataphysics OCA 15EC; Nicomp 380 zeta potential analyzer

Individual Files: