Abstract:

This project investigated the effect of surfactant chemistry and concentration on the initial adhesion of Marinobacter hydrocarbonoclasticus at the interface of dodecane droplets suspended in water, investigating both the time-dependent adhesion of bacteria at the dodecane/water interface and the dependence of adhesion on droplet size and surfactant concentration. The dataset consists of images (.bmp) for dodecane-in-water emulsions prepared using co-flow microfluidic device, z-stack images (.tiff) for three different drop sizes at different time intervals, z-stack images (.tiff) for two different drop sizes at different surfactant concentrations and pendant drop movies (.avi files) at different surfactant concentrations for the dodecane/water system. The data analyzed consists of: (1) normalized drop diameter as a function of inner to outer fluid flow rates in microfluidic device, (2) number of cell and cells normalized with drop surface area as a function of time, (3) time constant of adhesion as a function of drop diameter, (4) orientation distribution of cells at dodecane/water interface for three drop sizes, (5) cell areal density as a function of surfactant concentration, (6) interfacial tension as a function of surfactant concentration, and (7) cell areal density as a function of interfacial tension.

Suggested Citation:

Jacinta C. Conrad, Narendra K. Dewangan. 2018. Adhesion of Marinobacter hydrocarbonoclasticus to surfactant-decorated oil droplets. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7CR5RZZ

Publications:

Dewangan, N. K., & Conrad, J. C. (2018). Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets. Langmuir, 34(46), 14012–14021. doi:10.1021/acs.langmuir.8b02071

Purpose:

The project aimed to understand how dispersants affect the adhesion of bacteria at oil/water interfaces. We examined the effect of various surfactant chemistries and concentration on adhesion by measuring number of cells adhering at oil/water interfaces using single cell tracking algorithms. We have also investigated the effect of drop size in bacterial adhesion.

Data Parameters and Units:

Concentration (ppm and g/l), Absorbance (a.u.), Interfacial tension (IFT, mN/m), Surface energy (mN/m), Flow rate (qi, qo, ul/min), Time (min), Drop diameter (um), Surface excess (mole/ m^2), Surface area per molecule (A^2), Salt concentration (M), Cell areal density (um^-2), Cell volume density (ul^-1); Number of cells (unitless), EG (ethylene glycol), DIM (diiodomethane), L (left contact angle in degrees of sessile drop), R (right contact angle in degrees of sessile drop), SE (surface energy in mN/m), U (electrophoretic mobility in m.u., microcm/V-s), Zeta (zeta potential in mV)

Individual Files: