Abstract:

These are photographs of HepG2 cells treated with nothing, dimethylsulfoxide (DMSO, solvent vehicle), chrysene, or its six monomethylated isomers after 5 days of exposure. The chrysenes caused flattened and elongated shape changes that may be consistent with epithelial to mesenchymal transition of the cells (EMT). This morphological change has been reported previously for epithelial cells that were exposed to polycyclic aromatic hydrocarbons. The chrysenes all have similar dose dependent effects on the HepG2 cells. Each photo is annotated in the title with the magnification (200x) the treatment [untreated, dimethylsulfoxide (DMSO) vehicle, chrysene or methyl chrysene isomer (1-6)] and the last number reflects the number of photo taken (1-4 photos from different microscopic fields).

Suggested Citation:

Ahmad Alquassim and Charles Miller. 2017. Altered morphology of HepG2 cells exposed to chrysene isomers. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7DZ06NS

Purpose:

Effects of chrysenes are being examined in HepG2 cells, which are a model for human liver hepatocytes.

Data Parameters and Units:

Magnification (200x), Treatments [untreated, dimethylsulfoxide (DMSO) vehicle, chrysene or methyl chrysene isomer (1-6)], 0.005 to 5 micromolar (uM) doses of each chrysene compound were used, Number of photo taken (1-4 photos from different microscopic fields)

Methods:

Images were photographed at 200x magnification on an inverted microscope.

Provenance and Historical References:

Others have reported similar effects when chemicals trigger aryl hydrocarbon receptor signaling, e.g. Oncogene (2009) 28, 3642–3651; doi:10.1038/onc.2009.224. This has not been previously reported for these chyrysene isomers.

Individual Files: