Abstract:

We evaluated the cytotoxicity of chrysene and its monomethylated isomers using HepG2 (human hepatoma) cells. The treatment groups were a control, solvent control (DMSO up to 0.1%), chrysene, and its 6 mono methyl isomers at 5 nM, 50 nM, 500 nM, and 5 uM (final concentrations). The assays were carried out in 24-well microplates with 3 technical replicates per treatment group. Cells were seeded at 10,000/well on day 0. Exposure time to treatments was 5 days. Sulforhodamine B fluorescence was measured on a Tecan microplate reader following the published SRB assay. All chrysenes were toxic at the 5 uM dose, and some toxicity (reduced cell density as indicated by sulforhodamine B dye binding) at the 500 nM dose as well. None were significantly toxic at 50 and 5 nM.

Suggested Citation:

Ahmad Alqassim, Charles Miller. 2017. Cytotoxicity of chrysene isomers to HepG2 cells. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7W094B1

Publications:

Alqassim, A. Y., Wilson, M. J., Wickliffe, J. K., Pangeni, D., Overton, E. B., & Miller, C. A. (2019). Aryl hydrocarbon receptor signaling, toxicity, and gene expression responses to mono‐methylchrysenes. Environmental Toxicology, 34(9), 992–1000. doi:10.1002/tox.22770

Purpose:

To determine cytotoxicity of chrysene isomers to HepG2 cells.

Data Parameters and Units:

This assay uses a spectrophotometer to measure sulforhodamine B dye binding as a reflection of cell density. Data are collected as absorbance units +/- standard deviation for triplicate samples per treatment. Treatment concentration (% or uM).

Methods:

We evaluated the cytotoxicity of chrysene and its monomethylated isomers using HepG2 (human hepatoma) cells. The treatment groups were a control, solvent control (DMSO up to 0.1%), chrysene, and its 6 mono methyl isomers at 5 nM, 50 nM, 500 nM, and 5 uM (final concentrations). The assays were carried out in 24-well microplates with 3 technical replicates per treatment group. Cells were seeded at 10,000/well on day 0. Exposure time to treatments was 5 days. Sulforhodamine B fluorescence was measured on a Tecan microplate reader following the published SRB assay. All chrysenes were toxic at the 5 uM dose, and some toxicity (reduced cell density as indicated by sulforhodamine B dye binding) at the 500 nM dose as well. None were significantly toxic at 50 and 5 nM.

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