Abstract:

The dataset contains sequences, NCBI accession details (NCBI BioProject: PRJNA530182; BioSample: SAMN11302943; Sample name: Candidatus Macondimonas diazotrophica strain:KTK01) and the sampling details including the location, depth and date of sample collection. This dataset supports the publication: Karthikeyan, S., Rodriguez-R, L. M., Heritier-Robbins, P., Kim, M., Overholt, W. A., Gaby, J. C., … Konstantinidis, K. T. (2019). “Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments. The ISME Journal. doi:10.1038/s41396-019-0400-5

Suggested Citation:

Smruthi Karthikeyan, Luis M Rodriguez-R, Patrick Heritier-Robbins, Minjae Kim, Will A Overholt, John C. Gaby, Janet K. Hatt, Jim C. Spain, Ramon Rosselló-Móra, Markus Huettel, Joel E Kostka, Konstantinos T Konstantinidis. 2019. Dataset for: “Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-vtmz-k816

Publications:

Karthikeyan, S., Rodriguez-R, L. M., Heritier-Robbins, P., Kim, M., Overholt, W. A., Gaby, J. C., … Konstantinidis, K. T. (2019). “Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments. The ISME Journal. doi:10.1038/s41396-019-0400-5

Purpose:

Description of a novel bacterial genus found in beach sands affected by Macondo Oil.

Data Parameters and Units:

Data Available at https://doi.org/10.1038/s41396-019-0400-5, NCBI BioProject PRJNA530182 The dataset contains sequences and location and date of sampling.

Methods:

DNA was extracted using the QIAmp DNA Mini kit (Qiagen, MD) according to the manufacturer’s protocol. DNA sequencing libraries were prepared using the Illumina Nextera XT DNA library prep kit according to manufacturer’s instructions except the protocol was terminated after isolation of cleaned double stranded libraries. Library concentrations were determined by fluorescent quantification using a Qubit HS DNA kit and Qubit 2.0 fluorometer (ThermoFisher Scientific) and samples run on a High Sensitivity DNA chip using the Bioanalyzer 2100 instrument (Agilent) to determine library insert sizes. The KTK-01 isolate genome was sequenced using a MiSeq reagent v2 kit for 500 cycles (2 x 250 bp paired end run) on an Illumina MiSeq instrument (located in the School of Biological Sciences, Georgia Institute of Technology). Adapter trimming and demultiplexing of the sequenced samples were carried out by the MiSeq Control Software.

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