Dataset Available

Sequences used in the phylogenetic analysis of flies in the family Tabanidae collected from coastal regions of Louisiana from 2016-07-13 to 2018-08-08

Authors: Davis, Debra, Jin Tao, Claudia Husseneder, Nathan P. Lord, and Lane D. Foil
Published On: May 02 2022 01:35 UTC
DOI: https://doi.org/10.7266/T8BNF6G4
UDI: R5.x277.000:0014
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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-V

Research Group:
A study of horse fly (Tabanidae) populations and their food web dynamics as indicators of the effects of environmental stress on coastal marsh health.

Point of Contact

Lane Foil
Louisiana State University Ag Center / Department of Entomology
lfoil@agcenter.lsu.edu

Data Collection Period
2016-07-13
to
2018-08-08
Theme keywords

Tabanus nigrovittatus, Tabanidae, Louisiana, Salt Marsh, flies, sequence, DNA, phylogenetic analysis, cryptic species

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Abstract:

Flies in the family Tabanidae were collected from coastal Louisiana using canopy traps baited with dry ice from 2016-07-13 to 2018-08-08. Tabanids were identified using the keys of Tidwell (1973). DNA was extracted from individuals of select species and portions of DNA from the 18s and CO1 regions were amplified using three primer sets and sequenced. The sequences were then submitted to NCBI GenBank. Phylogenetic trees were built using these sequences.

Suggested Citation:

Davis, Debra, Jin Tao, Claudia Husseneder, Nathan P. Lord, and Lane D. Foil. 2022. Sequences used in the phylogenetic analysis of flies in the family Tabanidae collected from coastal regions of Louisiana from 2016-07-13 to 2018-08-08. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/T8BNF6G4

Purpose:

Individuals from select species of Family Tabanidae from coastal Louisiana were collected and sequenced in order to phylogenetically compare populations and to determine the possibility of cryptic species.

Data Parameters and Units:

Species, Collection Date, Location, Gene, Primer Pair, Accession Number.

Methods:

Flies in the family Tabanidae were collected from coastal Louisiana using canopy traps baited with dry ice from 2016-07-13 to 2018-08-08. Tabanids were identified using the keys of Tidwell (1973). DNA was then extracted from individuals of select species and portions of DNA from the 18s and CO1 regions were amplified using three primer sets (ai/bi, NF1/18Sr2b, and LCO1490/HCO2198) and sequenced.

Provenance and Historical References:

Tidwell, M.A. 1973. The Tabanidae (Diptera) of Louisiana. Tulane Studies in Zoology and Botany 18, 1-95.

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