Dataset Available

18S metagenomic sequencing of DNA isolated from sediments from larval collection sites (oiled and non-oiled locations) and tabanid larval gut contents from coastal Louisiana, 2011-08-25 to 2011-09-28

Authors: Devika Bhalerao
Published On: Feb 28 2020 20:56 UTC
DOI: https://doi.org/10.7266/n7-f9y9-f494
UDI: R5.x277.000:0006
Files

Individual Files

No. of Downloads: 2

No. of Files: 1

File Size: 23.33 KB

File Format(s):
xlsx

Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-V

Research Group:
A study of horse fly (Tabanidae) populations and their food web dynamics as indicators of the effects of environmental stress on coastal marsh health.

Point of Contact

Lane Foil
Louisiana State University Ag Center / Department of Entomology
lfoil@agcenter.lsu.edu

Data Collection Period
2011-08-25
to
2011-09-28
Theme keywords

Horse fly larvae, Tabanid larvae, Greenhead horse fly, Tabanid larva, sediment DNA, sediment metagenome, insect gut metagenome

ISO 19115-2 Metadata
  View Metadata
Abstract:

DNA extracted from sediments and tabanid larval gut contents were subjected to 18SrRNA gene amplification and high-throughput Illumina sequencing. Sequences assembled into contigs of operational taxonomic units (greater than 97% match for homology in the environmental genome) has been submitted to GenBank. Raw Illumina Miseq sequence data for the 18SrRNA gene for all BioSamples have been deposited in the Sequence Read Archive (SRA) under BioProject PRJNA408157 and have been released with SRA study number SRP118538 titled “18S metabarcoding of greenhead horse fly food web: Larval gut contents and sediments.” in the NCBI database. Biosamples contain collection information including geospatial location and date.

Suggested Citation:

Devika Bhalerao. 2020. 18S metagenomic sequencing of DNA isolated from sediments from larval collection sites (oiled and non-oiled locations) and tabanid larval gut contents from coastal Louisiana, 2011-08-25 to 2011-09-28. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-f9y9-f494

Publications:

Husseneder, C., Bhalerao, D. R., & Foil, L. D. (2022). Was the decline of saltmarsh tabanid populations after the 2010 oil spill associated with change in the larval food web? Ecosphere, 13(7). Portico. https://doi.org/10.1002/ecs2.4157

Purpose:

To identify the food web of the tabanid larvae to determine bioindicators of marsh health.

Data Parameters and Units:

NCBI SRA# (SRS2530606), BioProject# (Bio project accession number; PRJNA408157), Sample type (Tabanid larva or Sediment DNA), Biosample (accession number), Sample name, Organism (insect gut 18S metagenome, insect gut tissue 18S metagenome, insect gut tissue 18S metagenome, sediment 18S metagenome), Package, Accession, ID, Collection Date (MM/DD/YYYY), Location, Latitude (decimal degrees), Longitude (decimal degrees), Altitude (sea level), tissue, Species, development stage, broad-scale environmental context, identified by, number of larvae in sediment, relationship to oxygen, sample collection device or method, sample material processing, DNA extraction sample size, DNA Extraction, Forward Primer, Reverse Primer, and Sequencing Platform (Illumina MiSeq).

Methods:

Illumina sequencing of 18S rRNA genes.

Instruments:

Illumina Miseq 2X300BP

Individual Files: