Abstract:

As part of a study to investigate the effects of seepage on corals and their microbial associates, the metatranscriptome of corals was sequenced to understand gene expression of their microbial associates. The dataset contains bioSample accession numbers of sequences, dates, depth, longitude and latitude of sample collection.

Suggested Citation:

Vohsen, Samuel, Iliana Baums, and Charles Fisher. 2021. Metatranscriptomes of deep-sea corals and their microbial associates. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/1C1JPA8B

Purpose:

To understand the physiology of microbes that associate with deep-sea corals.

Data Parameters and Units:

The dataset contains the following headers- accession, BioSample ID, sample_name, sample_title, bioproject_accession, organism, collection_date, env_local_scale, coral species, depth (m), colony_name, latitude, longitude, Preservation, rRNA depleted and Site.

Methods:

RNA was extracted from samples of Callogorgia delta and Paramuricea sp. B3 using RNA extraction kits. Select samples were enriched for microbial RNA by removing host rRNA using a Ribozero Gold kit designed for yeast. RNA was sequenced on an Illumina MiSeq platform using 100bp single-end reads.

Instruments:

Illumina MiSeq platform

Individual Files: