Abstract:

This study examined how the interaction of oil with clays and or marine snow affected the biodegradation of oil by specific bacteria (Pseudomonas putida F1 and Rhodococcus qingshengii TUHH‌-12). This dataset contains experimental data on the effect of Corexit, EPS and suspended particles on the degradation of crude oil (oxygen consumption, n-alkane and BTEX and PAHs biodegradation). This dataset supports the publication: Rahsepar, S., Langenhoff, A. A. M., Smit, M. P. J., van Eenennaam, J. S., Murk, A. J., & Rijnaarts, H. H. M. (2017). Oil biodegradation: Interactions of artificial marine snow, clay particles, oil and Corexit. Marine Pollution Bulletin, 125(1-2), 186–191. doi:10.1016/j.marpolbul.2017.08.021

Suggested Citation:

Shokouh Rahsepar. 2019. Dataset for: Oil biodegradation: Interactions of artificial marine snow, clay particles, oil and Corexit. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-5e1z-nf25

Publications:

Rahsepar, S., Langenhoff, A. A. M., Smit, M. P. J., van Eenennaam, J. S., Murk, A. J., & Rijnaarts, H. H. M. (2017). Oil biodegradation: Interactions of artificial marine snow, clay particles, oil and Corexit. Marine Pollution Bulletin, 125(1-2), 186–191. doi:10.1016/j.marpolbul.2017.08.021

Purpose:

The purpose is to understand the interaction between Corexit, suspended particles and EPS and their effects on oil biodegradation.

Data Parameters and Units:

Information worksheet: Includes information on the growth medium used in the experiment, concentrations of oil and Corexit EC9500A, bacterial culture, and kaolin clay. Table 1 of manuscript worksheet: Exposures (Oil+Snow, Oil+Clay, Oil+Snow+Clay, Oil only); ΣBTEX (µg/L) in absence of Corexit; ΣBTEX (µg/L) in presence of Corexit. Particle size distribution worksheet: Name of the condition {Oil+Snow+Clay+Corexit, Oil+Snow+Clay, Oil+Snow, Snow+Clay (no oil), Snow only (no oil), Oil+Clay+Corexit, Oil+Clay, Clay only (no oil), Oil+Corexit, Oil+Snow+Corexit}; Volume of Particles (%); Particle size (µm). Cumulative oxygen consumption worksheet: Name of the condition {Oil+Clay, Oil+Snow+Clay, Oil+Snow+Corexit, Oil+Clay+Corexit, Oil+Snow+Clay+Corexit, Snow only (no oil), Snow+Clay (no oil)}; Cumulative O2 consumption (mmol); Time (day); n.a = not available. Relative n-alkane peak area worksheet: Name of the condition (Oil+Snow, Oil+Clay, Oil+Snow+Clay, Oil+Snow+Corexit, Oil+Clay+Corexit, Oil+Snow+Clay+Corexit); Relative n-alkane peak area; Time (day); n.a = not available. Relative BTEX peak area worksheet: Name of the condition (Oil+Snow, Oil+Clay, Oil+Snow+Clay, Oil+Snow+Corexit, Oil+Clay+Corexit, Oil+Snow+Clay+Corexit); Relative BTEX peak area; Time (day); n.a = not available.

Methods:

In this experiment, bacterial cultures were exposed to a variety of conditions (Oil + Snow, Oil + Clay and Oil+ Snow + Clay) with and without Corexit. Abiotic controls were simultaneously set up. Macondo surrogate oil (MC252) was used in this study. The initial concentration of oil was 0.1g of oil per 20mL of medium. Corexit® EC9500A (Nalco Holding Company, USA) was applied as a chemical dispersant. DOR (dispersant to oil ratio) was 1:20 in this study. At the beginning of the experiments, the optical density of Pseudomonas putida F1 (DSMZ, No. 6899) was 0.305 and the optical density of Rhodococcus qingshengii TUHH‌-12 (DSMZ No. 46766) was 0.98. Kaolin clay (hydrated aluminium silicate, CAS 1332-58-7, Sigma Aldrich) with kaolin clay:oil ratio (KOR) 1:2.6 was added in the conditions containing kaolin clay. Batch experimental bottles contained 20 mL bacterial growth medium, in artificial sea water (32 g/L artificial sea salt in demi-water). The growth medium consisted of (per litre of water) 10.4g Na2HPO4; 5.32g KH2PO4; 4g (NH4)2SO4; 0.8g MgSO4.7H2O; 1mL of trace element solution (2g/L FeCl3.4H2O; CoCl2.6 H2O 2g; 1g/L CaCl2.2H2O; 0.5g/L MnCl2.4H2O; 30mg/L CuCl2.2H2O; ZnCl2 50mg/L; 50mg/L HBO3; 90mg/L (NH4)6Mo7O24.4H2O; 100mg/L Na2SeO3.5H2O; 50mg/L NiCl2.6H2O; 1g/L EDTA; 1mL/L 36% HCl); resazurin 0.5g/L, and 1mL of vitamin solution (0.106mg/L biotin; 0.005mg/L folic acid; 0.0025mg/L pyridoxal-HCl; 0.015mg/L lipoic acid; 0.0125mg/L riboflavin; 0.266mg/L thiamine-HCl; 0.413mg/L Ca-D-pantothenate; 0.0125mg/L cyanocobalamin; 0.0125mg/L p-aminobenzoic acid; 0.0125mg/L nicotinic acid). Total Petroleum Hydrocarbons (TPH) were extracted from the liquid phase according to liquid-liquid extraction protocol NEN 5733 and analyzed using GC-FID (Fisions 8000). The liquid-liquid extraction protocol NEN 5733 was used to remove and concentrate Total Petroleum Hydrocarbons (TPH). A GC-FID (Fisions 8000) was used to quantify Total Petroleum Hydrocarbons. Details on liquid-liquid extraction protocol NEN 5733 can be found at Rahsepar et.,2016. BTEX compounds (benzene, toluene, ethylbenzene and o-, m-, and pxylene) were extracted from the headspace of the batches by solid phase micro extraction (SPME) and were analyzed on a GC-FID. Solid phase micro extraction (SPME) was used to extract BTEX compounds (benzene, toluene, ethylbenzene and o-, m-, and pxylene) from the headspace. GC-FID was used in the analysis of BTEX compounds.

Instruments:

GC-FID Gas chromatography with flame ionization detector manufactured by Shimadzu. A GC-FID (Fisions 8000).

Provenance and Historical References:

Rahsepar, S., Smit, M. P. J., Murk, A. J., Rijnaarts, H. H. M., & Langenhoff, A. A. M. (2016). Chemical dispersants: Oil biodegradation friend or foe? Marine Pollution Bulletin, 108(1-2), 113–119. doi:10.1016/j.marpolbul.2016.04.044

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