Abstract:

This study quantifies the growth of Alcanivorax and Acinetobacter on crude and dispersed oil, changes in composition that Alcanivorax and Acinetobacter have of crude and dispersed oil, and the impact that Alcanivorax and Acinetobacter have on oil ecotoxicity. Components are divided into three main groups, (1) bacterial growth rates, (2) changes to total petroleum hydrocarbons and specific hydrocarbon fractions following bacterial growth, and (3) the ecotoxicity associated with water-entrained hydrocarbons following bacterial growth. This dataset corresponds with the publication "Hydrocarbon Degrading Bacteria Exhibit a Species Specific Response to Dispersed Oil while Moderating Ecotoxicity" (Overholt, Will A., et al. ). Applied and Environmental Microbiology (2015): AEM-02379.

Suggested Citation:

Overholt, Will and Kostka, Joel. 2016. Lab study on Alcanivorax and Acinetobacter growing on crude and dispersed oil: Growth rates, changes in oil composition, and oil ecotoxicity. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N70P0X2C

Publications:

Overholt, W. A., Marks, K. P., Romero, I. C., Hollander, D. J., Snell, T. W., & Kostka, J. E. (2015). Hydrocarbon-Degrading Bacteria Exhibit a Species-Specific Response to Dispersed Oil while Moderating Ecotoxicity. Appl. Environ. Microbiol., 82(2), 518–527. doi:10.1128/aem.02379-15

Purpose:

The objectives of this study were (i) to identify and characterize predominant oil-degrading taxa that may be used as model hydrocarbon degraders or as microbial indicators of contamination and (ii) to characterize the in situ response of indigenous bacterial communities to oil contamination in beach ecosystems.

Data Parameters and Units:

Below is a list of data parameters and their units of measurement for each file in the dataset: Acinetobacter_growth.csv: Total protein concentration data for Acinetobacter strain COS-3 grown with oil, oil+corexit, or corexit alone as sole carbon sources. Total protein concentrations represent the mean from triplicate samples, standard deviation is included. Two separate controls were used, an inoculated control with no bacteria, and an inoculated control with no carbon source. Dataset fields: Hour (0, 18, 42, 90, 120), Protein concentration (ug/ml), Treatment [Uninoculated Control, Corexit (0.01% COREXIT 9500A), Oil (0.5% crude oil), Inoculated Control, Corexit-oil (1:50 COREXIT 9500A:oil mixture)], Standard Deviation; Alcanivorax_growth.csv: Total protein concentration data for Alcanivorax strain P2S70 grown with oil, oil+corexit, or corexit alone as sole carbon sources. Total protein concentrations represent the mean from triplicate samples, standard deviation is included. Two separate controls were used, an inoculated control with no bacteria, and an inoculated control with no carbon source. Dataset fields: Hour (0, 18, 42, 90, 120), Protein concentration (ug/ml), Treatment [Uninoculated Control, Corexit (0.01% COREXIT 9500A), Oil (0.5% crude oil), Inoculated Control, Corexit-oil (1:50 COREXIT 9500A:oil mixture)], Standard Deviation; oil_deg_concentrations.csv: Concentration of total petroleum hydrocarbons in culture media sacrificed after 7 and 14 days. Concentration was corrected for extraction efficiency using percent surrogate recovered. Concentration/mg was normalized by mass of crude oil added. Dataset fields: Time (7d, 14d), Bacteria (Control, Alcanivorax, Acinetobacter), Treatment [Oil (0.5% crude oil), dispersed oil (1:50 COREXIT 9500A:oil mixture)], Replicate (Control A, Control B, Control C, P2-S70 A, P2-S70 B, P2-S70 C, COS-2 A, COS-2 B, COS-2 C), Area (no unit of measurement), Concentration (total petroleum hydrocarbons (ppm) in culture media sacrificed after 7 and 14 days), Corrected_concentration (total petroleum hydrocarbons (ppm) in culture media sacrificed after 7 and 14 days corrected for extraction efficiency using percent surrogate recovered), Concentration/mg (total petroleum hydrocarbons (ppm per mg oil added) in culture media sacrificed after 7 and 14 days normalized by mass of crude oil added); survival_curves_corexit_only.csv: Acute toxicity data showing number of rotifers that survived for each treatment/dilution. Results after 24 and 48 hours shown. Dataset fields: Concentration [concentration percentage of 0.01% COREXIT 9500A in each sample (0, 20, 40, 60, 80, 100)], Survivorship (percentage of rotifer survival), Treatment (Control, Alcanivorax, Acinetobacter), Time [hours (24, 48)], SE (standard error), Mortality (percentage of rotifer mortality), Concentration [corexit concentration (ug/ml)]; survival_curves_oil_only.csv: Acute toxicity data showing number of rotifers that survived for each treatment/dilution. Results after 24 and 48 hours shown. Dataset fields: Concentration [concentration percentage of 0.5% (vol/vol) Macondo surrogate crude oil (0, 20, 40, 60, 80, 100)], Survivorship (percentage of rotifer survival), Treatment (Control, Alcanivorax, Acinetobacter), Time [hours (24, 48)], SE (standard error), Mortality (percentage of rotifer mortality); survival_curves_oil+corexit.csv: Acute toxicity data showing number of rotifers that survived for each treatment/dilution. Results after 24 and 48 hours shown. Dataset fields: Concentration [concentration percentage of 1:50 Corexit 9500A-dispersed Macondo crude oil (0, 20, 40, 60, 80, 100)], Survivorship (percentage of rotifer survival), Treatment (Control, Alcanivorax, Acinetobacter), Time [hours (24, 48)], SE (standard error), Mortality (percentage of rotifer mortality); stir_shake_waf_test.txt: Experiment to determine whether we could generate WAF using a shaker table instead of the industry standard stirring method. Results are presented in total number of rotifers tested and the number that died. Dataset fields: Treatment (Oil + Corexit), Time (18hr, 7d), Method (stir, shake), [concentration percentage of 1:50 Corexit 9500A-dispersed Macondo crude oil (0, 20, 40, 60, 80, 100)], total rotifers, mortality; WAF_aliphatics.txt: Concentration of aliphatics entrained in the culture media. Results are in ug/L. Dataset fields: Sample ID (Control+oil, Alcanivorax+oil, Acinetobacter+oil), Species (Control, Alcanivorax, Acinetobacter), Treatment (0.5% (v/v) Oil, 1:50 Corexit® 9500A:Oil), Mass oil added (mg), Sample Type (WAF) , The remaining fields represent the concentration values for each aliphatic hydrocarbon measured in ug/L (C12-C17, Pristane, C18, Phytane, C19-C40); WAF_armoatics.txt: Concentration of aromatics entrained in the culture media. Results are in ug/L. Dataset fields: Sample ID (Control+oil, Alcanivorax+oil, Acinetobacter+oil, EPA), Species (Control, Alcanivorax, Acinetobacter), Treatment (0.5% (v/v) Oil, 1:50 Corexit® 9500A:Oil), Mass oil added (mg), Sample Type (WAF, acute potency divisor limits) , The remaining fields represent the concentration values for each aromatic hydrocarbon measured in ug/L (N, N1-N5, ACL, ACE, F, AN, P, P/AN1, P/AN2-P/AN5, D, D1, D2, FL, PY, FL/PY1- FL/PY5, BAA, C, BAA/C1- BAA/C5, BBF, BKF, BAP, Pe, ID, DA, BGP, BP/PER1-BP/PER5).

Individual Files: