GRIIDC | R4.x267.000:0104

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Nitrogen fixing microbial communities in oiled and clean sands of Pensacola Beach, July 2010 and June 2011, and sugarcane and peat samples

Authors: Kostka, Joel & Rishishwar, Lavanya

Published On: Jul 09 2018 19:54 UTC

DOI: https://doi.org/10.7266/N7PV6J04

UDI: R4.x267.000:0104

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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-IV

Research Group:
Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

Point of Contact

Joel E. Kostka
Georgia Institute of Technology / School of Earth and Atmospheric Sciences
joel.kostka@biology.gatech.edu

Data Collection Period

2010-07-30

2011-07-14

Theme keywords

nitrogen-fixing microbial communities, PCR amplification, nitrogenase gene, nifH

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Abstract:

These data report the diversity and composition of nitrogen-fixing microbial communities sampled from beach sands collected from Pensacola Beach. Nitrogen-fixers were characterized by the extraction, PCR amplification, and next generation sequencing of the nitrogenase gene (nifH). Sugarcane and peat samples were also analyzed.

Suggested Citation:

Kostka, Joel & Rishishwar, Lavanya. 2018. Nitrogen fixing microbial communities in oiled and clean sands of Pensacola Beach, July 2010 and June 2011, and sugarcane and peat samples. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7PV6J04

Publications:

Gaby, J. C., Rishishwar, L., Valderrama-Aguirre, L. C., Green, S. J., Valderrama-Aguirre, A., Jordan, I. K., & Kostka, J. E. (2017). Diazotroph Community Characterization via a High-Throughput nifH Amplicon Sequencing and Analysis Pipeline. Applied and Environmental Microbiology, 84(4), e01512–17. doi:10.1128/aem.01512-17

Purpose:

To report the diversity and composition of nitrogen-fixing microbial communities sampled from beach sands collected from Pensacola Beach.

Data Parameters and Units:

Multiplexed libraries containing nifH sequence operational taxonomic unit identified to lowest taxanomic level (counts per sample), primername, barcode name, barcode sequence.

Methods:

Oiled and clean sand samples were collected from Pensacola Beach, FL, USA in July 2010 and June 2011, respectively. Peat samples were collected near Grand Rapids, MN, USA in July 2012. Sugarcane samples were collected in Liberia. Sample equipment: hand coring, grab samples.