Abstract:

The seawater samples were collected from Pensacola Beach, FL in June and August, 2016. The seawater was incubated with low concentration of oil (2ppm) with or without dispersant. Oil analysis and sequencing data were generated.

Suggested Citation:

Lena Chu, Elisa Mercando, Roger C Prince, Isabel Romero David Hollander. 2018. Dispersant enhances hydrocarbon degradation and alters the structure of metabolically active microbial communities in shallow seawater from the northeastern Gulf of Mexico. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N72V2DNW

Publications:

Sun, X., Chu, L., Mercando, E., Romero, I., Hollander, D., & Kostka, J. E. (2019). Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico. Frontiers in Microbiology, 10. doi:10.3389/fmicb.2019.02387

Purpose:

The data were collected in order to assess the effect of dispersant application during oil spills.

Data Parameters and Units:

Respiration data (umol O2/L seawater/Day), Respiration data (umol CO2/L seawater/Day), Oil concentration (ppm), Concentration (ng/L) high-mixing alkanes, Concentration (ng/L) high-mixing PAHs, Raw measurement low mixing PAH, Hopane normalized low mixing PAHs, Percent loss low mixing PAHs, Raw measurement low mixing alkanes, Hopane normalized low mixing alkanes, Percent loss low mixing alkanes, Timepoint (days), Treatment, Sequencing data: BioSample, Carbon (dispersant or oil), Experiment, Identification, Library Name, MBases, MBytes, Replicate, Run, SRA Sample, Sample Name, Time, Assay Type (AMPLICON), Avg Spot Len (501), BioProject, Bio Sample Model (Metagenome or environmental), Consent (public), Insert Size (0), Instrument (Illumina MiSeq), Library Layout (SINGLE), Library Selection (PCR), Library Source (METAGENOMIC), Load Date (MM/DD/YYYY), Organism (seawater metagenome), Platform (ILLUMINA), Release Date (MM/DD/YYYY), SRA Study (SRP133151), Collection date (MM/DD/YYYY), Geo_loc_name (USA: Pensacola Beach), Host (N/a), Isolation_source (Pensacola beach), Latitude longitude (lat_lon, decimal degrees)

Methods:

Seawater collected from Pensacola Beach, Florida was incubated with a low concentration of oil (2 ppm, 3 uL of oil was added to 1.5 L of seawater) or dispersant and oil with a ratio of 1:15 or 0.2 uL of dispersant. The dispersant used with Surrogate MC252 oil was weathered by evaporation in the lab to approximately 20 % weight loss. The surrogate oil was characterized in Pelz, O.; Brown, J.; Huddleston, M.; Rand, G.; Gardinali, P.; Stubblefield, W.; Benkinney, M. T.; Ahnell, A. Selection of a Surrogate MC252 Oil as a Reference Material for Future Aquatic Toxicity Tests and Other Studies. Poster presented at: Society of Environmental Toxicology and Chemistry, 2011 Nov 13-17, Boston, Massachusetts, USA. Concentrations of low mixing alkanes, low mixing PAHs, high mixing alkanes, and high mixing PAHs were measured after 0, 7, 14, 21, 30 and 40 days. Additional bottles were capped with rubber stoppers for quantification of respiration. Oxygen concentrations were measured by a Presens Microx 4 with PSt7 non-invasive sensor spots (Presens, Germany) adhered to the inside of the glass bottle. Carbon dioxide concentrations were measured by injecting 100 µl of headspace into a GC-FID equipped with a methanizer (Shimadzu, Japan).

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